Picture from Zheng Q et al. (2014) PLoS Genet "The vesicle protein SAM-4 regulates the processivity of synaptic vesicle ...."

Figure 2. sam-4 encodes a neuronally expressed protein SV associated protein. (A-C9) Distribution of SAM-4-TagRFP expressed in mechanosensory neurons in the synaptic varicosities (A) and the PLM soma and proximal section of the PLM neurite (C). GFP-RAB-3 localization is also shown in (B and B9) along with the merge (C and C9). (D-E) Western blots of fractionated whole worm extracts (D) and non-fractionated lysis (E) derived from animals expressing 3XFlag tagged SAM-4(+) and SAM-4(G2S) myristoylation mutant proteins probed for SAM-4, the synaptic vesicle protein SNB-1, and the cytosolic protein b-Tubulin. Note the shift in migration of the SAM-4(G2S) mutant. (F) Quantification of GFP-RAB-3 levels in PLM synaptic varicosities. **, P,0.001, relative to wild type; DD, P,0.001, relative to sam-4. Transgenes tested: jsIs1188 for sam-4(+), jsIs1265 for sam4(G2S). Allele tested: sam-4(js415). (G-H9) SAM-4(G2S)-TagRFP (jsEx1256) levels are reduced in PLM synaptic varicosities (G and H) but increased in PLM soma (G9 and H9), relative to SAM-4-TagRFP (jsIs1156). Solid arrow: PLM soma; open arrow: PLM neurite; solid arrowhead: PLM synaptic varicosity; open arrowhead: florescence marker puncta in PLM neurite; asterisk: vulva. Scale bars: 10 um.

Picture from Zheng Q et al. (2014) PLoS Genet "The vesicle protein SAM-4 regulates the processivity of synaptic vesicle ...."

Figure 2. sam-4 encodes a neuronally expressed protein SV associated protein. (A-C9) Distribution of SAM-4-TagRFP expressed in mechanosensory neurons in the synaptic varicosities (A) and the PLM soma and proximal section of the PLM neurite (C). GFP-RAB-3 localization is also shown in (B and B9) along with the merge (C and C9).

Picture from Niwa S et al. (2017) Curr Biol "BORC Regulates the Axonal Transport of Synaptic Vesicle Precursors by ...."

Figure 1. SAM-4 and ARL-8 Are in the Same Pathway(A) A schematic drawing of the DA9 neuron. The dorsal asynaptic region, the ventral axon, and the commissure that are observed and analyzed in this study areshown. Asterisk indicates the location where the commissure joins the dorsal nerve cord.(B-F) Representative images of GFP::RAB-3 in wild-type (WT) (B), sam-4(tm3828) (C), arl-8(tm2388) (D), arl-8(wy271) (E), and arl-8(wy271); sam-4(tm3828) (F).GFP::RAB-3 was expressed using the itr-1 pB promoter. Asterisks indicate the commissure bend shown in (A). Scale bars, 50 um.(G) Image montages of the dorsal axons in WT, sam-4(3828), arl-8(tm2388), arl-8(wy271), and sam-4(tm3828); arl-8(wy271). 10 confocal images from eachgenotype were cropped, straightened, and aligned in rows with the commissure bend (asterisk) on the right using ImageJ. Scale bar, 20 um.(H and I) Statistical analysis of mutant phenotypes. The number of puncta mis-accumulated to the commissure (H) and the length of the asynaptic region (I) areshown. Lines show median values, and each dot represents one animal. Kruskal-Wallis one-way ANOVA on ranks and Dunn's multiple comparisons test,*adjusted p value < 0.05, **adjusted p value < 0.01, and ***adjusted p value < 0.001; n = 20 animals for each genotype. See also Figure S1.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(g-h) vrk-1::gfp was expressed in several cells, which included head neurons (g) and phasmids (h).

Picture from Yu S et al. (1997) Proc Natl Acad Sci U S A "Guanylyl cyclase expression in specific sensory neurons: a new family of ...."

(G) Expression pattern of gcy-33::GFP.

Picture from Cuppen E et al. (2003) Comp Funct Genomics "Proteins interacting with Caenorhabditis elegans Galpha subunits."

Expression pattern of C01H6.9 (haspin; G) as determined by promoter-GFP reporter constructs. (G) C01H6.9 expression is shown in one of the phasmid neurons, PHB.

Picture from David DC et al. (2010) PLoS Biol "Widespread protein aggregation as an inherent part of aging in C. ...."

Pkin-19::kin-19::tagrfp animals (F and G).

Picture from Yang Y et al. (2017) Dev Cell "Spectraplakin Induces Positive Feedback between Fusogens and the Actin ...."

(E-G) Fluorescence time-lapse images of GFP-tagged EFF-1 (E), F-actin (F), and VAB-10A (G) during the seam-hyp7 cell fusion. Asterisks, V.a.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(G) Pgrd-12::GFP is expressed in two epithelial cells.

Picture from Lee HC et al. (2008) Mol Biol Cell "Caenorhabditis elegans mboa-7, a member of the MBOAT family, is required for ...."

Expression of MBOA-7::GFP in an early embryo (D and E) and a second-stage larva (F and G). Nomarski micrographs (D and F) and corresponding MBOA-7::GFP expression (E and G). MBOA-7::GFP was ubiquitously expressed throughout development. Asterisks indicate the anterior of the larva (F and G). Bar, 10 um (D and E) and 200 um (F and G).