Picture from Grosshans H et al. (2005) Dev Cell "The temporal patterning microRNA let-7 regulates several transcription ...."

Figure 4. The pha-4 3'UTR Regulates let-7-Dependent Downregulation of Intestinal pha-4::gfp Expression. (A) Examples of LCS:let-7 duplexes predicted for pha-4. (B and D) A pha-4::gfp::pha-43'UTR transgene is highly expressed in the intestinal cells of early stage (L3) larvae but is only weakly visible in L4 and adult animals. (C) Strong intestinal expression persists in adult animals when the pha-4 3'UTR of the transgene is replaced by the unc-54 3'UTR. (E) let-7(n2853) mutant animals continue to express the pha-4::gfp::pha3'UTR transgene as adults. Images in (D) and (E) were taken for the same exposure time and processed identically.

Picture from Grosshans H et al. (2005) Dev Cell "The temporal patterning microRNA let-7 regulates several transcription ...."

(B and D) A pha-4::gfp::pha-43'UTR transgene is highly expressed in the intestinal cells of early stage (L3) larvae but is only weakly visible in L4 and adult animals.

Picture from Dong X et al. (2013) Cell "An extracellular adhesion molecule complex patterns dendritic branching and ...."

(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(g-h) vrk-1::gfp was expressed in several cells, which included head neurons (g) and phasmids (h).

Picture from Fisher J et al. (2007) PLoS Comput Biol "Predictive modeling of signaling crosstalk during C. elegans vulval ...."

As examples for the time-course analysis, a mid-L2 larva at +22 h (A') and a late L2 larva at +28 h (B') are shown.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(H) Pgrl-27::GFP is expressed in arcade cells.

Picture from Yu S et al. (1997) Proc Natl Acad Sci U S A "Guanylyl cyclase expression in specific sensory neurons: a new family of ...."

(H 1-3) Expression pattern of gcy-32::GFP.

Picture from Fisher J et al. (2007) PLoS Comput Biol "Predictive modeling of signaling crosstalk during C. elegans vulval ...."

As examples for the time-course analysis, a mid-L2 larva at +22 h (A'') and a late L2 larva at +28 h (B'') are shown.

Picture from Cuppen E et al. (2003) Comp Funct Genomics "Proteins interacting with Caenorhabditis elegans Galpha subunits."

Expression pattern of T22A3.3 (H) as determined by promoter-GFP reporter constructs. (H) Expression of T22A3.3 is seen in a set of neurons in the head, including AWB.

Picture from Tepper RG et al. (2013) Cell "PQM-1 complements DAF-16 as a key transcriptional regulator of DAF-2-mediated ...."

(F-H) DAF-16 becomes more cytoplasmic with age, and nuclear localization of PQM-1::GFP decreases significantly with age. Data are represented as mean +/- SEM (G and H).