Picture from Orsborn AM et al. (2007) Development "GLH-1, the C. elegans P granule protein, is controlled by the JNK KGB-1 and by the ...."

KGB-1 is uniformly cytoplasmic in the distal gonad, aggregating into dispersed particles when oocytes cellularize. (A) A splayed gonad of a young N2 adult C. elegans (DAPI-stained). (B) The gonad in A reacted with anti-GLH-1 (red). (C) The gonad in A, with anti-KGB-1 (green). (D) A merged image of B and C. (E) Magnified view (1000x) of maturing oocytes (the boxed region from D).

Picture from Kawai H et al. (2009) Mol Biol Cell "Normal formation of a subset of intestinal granules in Caenorhabditis elegans ...."

(D-F) Staining of Is[haf-9::GFP] transgenic worms with acridine orange, a dye for acidic compartment labeling. GFP in green (D), acridine orange in magenta (E), and the merged images (F). Fluorescence from GFP and acridine orange is unmixed by lambda-scanning with a confocal microscope. HAF-9::GFP-positive granules do not overlap with acridine orange-positive granules. (J-L) Coexpression of HAF-9::GFP (J) with organelle marker LMP-1::mRFP (K), which is a C. elegans homologue of a mammalian lysosomal protein LAMP. As shown in the merged image (L), HAF-9::GFP is mostly colocalized with LMP-1::mRFP.

Picture from Kawai H et al. (2009) Mol Biol Cell "Normal formation of a subset of intestinal granules in Caenorhabditis elegans ...."

(D-F) Staining of Is[haf-9::GFP] transgenic worms with acridine orange, a dye for acidic compartment labeling. GFP in green (D), acridine orange in magenta (E), and the merged images (F). Fluorescence from GFP and acridine orange is unmixed by lambda-scanning with a confocal microscope. HAF-9::GFP-positive granules do not overlap with acridine orange-positive granules. (J-L) Coexpression of HAF-9::GFP (J) with organelle marker LMP-1::mRFP (K), which is a C. elegans homologue of a mammalian lysosomal protein LAMP. As shown in the merged image (L), HAF-9::GFP is mostly colocalized with LMP-1::mRFP.

Picture from Nair T et al. (2024) MicroPubl Biol "SKN-1 isoform-c is essential for C. elegans development."

Figure 1. SKN-1 isoform c is essential for embryonic development: (A) Schematic of skn-1 isoforms marking the location of the skn-1a Met1Ala ("no A") and skn-1c Met1Ala ("no C") mutations. (B) Quantification of percentage of plates that have viable progeny on them after 72 hours. (C) Quantification of embryonic development of embryos 15 hours post-egg lay. Percent of population categorized into 3 groups: less than 1.5-fold (blue), greater than 1.5-fold (red), and larval stage 1 (green). (D-E) Imaging of embryos derived from parents of the indicated genotypes (skn-1a Met1Ala, skn-1c Met1Ala, or skn-1(ok2315)); nT1 balancer harbors an integrated GFP marker to follow presence of the element.

Picture from Mathews EA et al. (2012) Genetics "Genetic interactions between UNC-17/VAChT and a novel transmembrane protein in ...."

Figure 4 SUP-1::CFP fusion is expressed in neurons and muscles. (A-C) Confocal images of a transgenic animal expressing wild-type SUP-1::CFP (green, A and B) and YFP::UNC-17 (magenta, B and C) fusion proteins under the control of their respective promoters. Shown is the head of a young adult hermaphrodite. The positions of the nerve ring (nr), dorsal nerve cord (dnc), and muscles are indicated. Bar, 10 um. (D-F) Confocal images of a transgenic animal expressing SUP-1::CFP (green, D and E) and cytochrome b5::YFP (an ER marker; magenta, E and F) in the ventral cord cholinergic motor neurons. SUP-1 is largely absent from the ER. Bar, 5 um. In all panels, anterior is to the left and ventral is down.

Picture from Walston T et al. (2004) Dev Cell "Multiple Wnt signaling pathways converge to orient the mitotic spindle in early ...."

Figure 2. KIN-19/CKI Localizes to Centrosomes and DSH-2 Accumulates between P2 and EMS(A-C) Localization of KIN-19 in the 4-cell embryo.(A) Antibody staining using an anti-human CKI antibody reveals KIN-19 localization at centrosomes during mitosis (arrows) and P granules (asterisk).(B) TBB-2/b-tubulin::GFP and HIS-11::GFP localize to microtubules and condensed chromosomes, respectively.(C) Merged image of anti-CKI (red) and TBB-2::GFP/HIS-11::GFP (both green).(D) In kin-19(RNAi) embryos, anti-CKI staining is not detectable at centrosomes and is reduced in P granules.(E) DSH-2 localizes to the cell cortex and is enriched at the boundary between P2 and EMS in the four-cell embryo (arrowheads). Scale bar equals 10 um.

Picture from Orsborn AM et al. (2007) Development "GLH-1, the C. elegans P granule protein, is controlled by the JNK KGB-1 and by the ...."

Figure 3. KGB-1 is uniformly cytoplasmic in the distal gonad, aggregating into dispersed particles when oocytes cellularize. (A) A splayed gonad of a young N2 adult C. elegans (DAPI-stained). (B) The gonad in A reacted with anti-GLH-1 (red). (C) The gonad in A, with anti-KGB-1 (green). (D) A merged image of B and C. (E) Magnified view (1000X) of maturing oocytes (the boxed region from D). (F) A splayed gonad from a young kgb-1(km21) worm (DAPI). (G) The gonad in F with anti-GLH-1. (H) The gonad in F with anti-KGB-1. The kgb-1(km21) strain (Mizuno et al., 2004) was used in F-H to establish the specificity of the N-terminal KGB-1 antibody as this region is deleted in kgb-1(km21), but not in kgb-1(um3).

Picture from Chuang CF et al. (2005) Genes Dev "A Toll-interleukin 1 repeat protein at the synapse specifies asymmetric odorant ...."

Figure 3. Subcellular localization of TIR-1. (A) Animals bearing the rescuing cosmid F13B10 were stained with TIR-1 antisera. TIR-1 staining is also visible in the intestine. (B) Animals expressing odr-3::tir-1 immunostained with TIR-1 antisera. White arrowhead indicates specific staining; open arrow and asterisk indicate nonspecific staining of the pharynx and buccal cavity, also seen with preimmune sera. (C) Confocal projections of animals expressing odr-3::tir-1::GFP false-colored green. (D) Confocal projections of animals expressing odr-3::tir-1::DsRed false-colored red. Cross-mark indicates autofluorescence. (E) Schematic representation of TIR-1::DsRed distribution pattern shown in D. (F,G) Animals expressing odr-3::tir-1deltaN1-444:: DsRed (F) or odr-3::tir-1deltaSAM::DsRed (G).

Picture from Grill B et al. (2007) Neuron "C. elegans RPM-1 regulates axon termination and synaptogenesis through the Rab ...."

Figure 2. GLO-4 Is Expressed in the Nervous System and Colocalizes with RPM-1. (A and B) Confocal images of adult animals expressing GLO-4::GFP costained with anti-GFP (green) and anti-SNT-1 (red in A), or anti-RPM-1 (red in B). All are wild-type animals except where indicated. GLO-4::GFP colocalized with RPM-1, but not SNT, in both SAB neurons and ventral nerve cord. The pattern of GLO-4::GFP in the ventral cord was unaltered in rpm-1-/-.(C) Confocal images of adult animals coexpressing Punc-25Cherry::GLO-4 (juEx1448) (red), and Punc-25RPM-1::GFP (juIs77) (green). (D) Confocal images of adult wild-type and glo-4-/- animals costained for endogenous RPM-1 (red) or endogenous RIM (green). RPM-1 localization was unaltered in glo-4-/-. Scale bars, 2 um.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(D, D') Pgrd-10::GFP is expressed in seam cells; the mid-body region is shown.