Picture from Qian X et al. (2024) MicroPubl Biol "Characterizing a standardized BioPart for BAG-specific expression in C. ...."

Figure 1. Expression and Characterization of Pflp-17 in BAG Neurons of C elegans:A. In hermaphrodites (top panel) and males (bottom panel) a Pflp-17::mScarlet transgene is expressed exclusively in BAG neurons (20x magnification, scale bars = 100 µm). B. Single-copy Pflp-17::mScarlet expression in a hermaphrodite (20x magnification, scale bar = 20 µm). C. flp-17 promoter characteristics. CenGEN data from https://cengen.shinyapps.io/CengenApp/ and https://cengen.textpressolab.com/. D. Schematic of standard mScarlet and gfp vectors for BAG-specific expression and identification. BsaI restriction sites allow exchanging the fluorescent reporter for a gene of interest by Golden Gate Assembly; ApaI (N-terminal) and SmaI (C-terminal) allow fluorophore fusions. The vectors are compatible with single-copy insertions (MosTI) and expression from extra-chromosomal arrays. E. Illustration of the transgenebuilder workflow. The short 300 bp Pflp-17 was added as a standard promoter in our online tool for optimizing transgene design and gene synthesis (www.wormbuilder.org/transgenebuilder).

Picture from Knoebel E et al. (2023) MicroPubl Biol "A toolkit for assembly of targeting clones for C. elegans ...."

Figure 1. Overview of Golden Gate (GG)-based targeting plasmid assembly:A) An RMCE vector and six insert-containing entry clones, each with distinct SapI generated 3 bp overlaps, are co-assembled using a SapI GG ligation protocol. The entry vector backbones are KanR (black) while the backbone of the targeting vector and final clone are AmpR (brown). In red is the designation given to each 'slot' with distinct SapI overhangs. B) PCR products (purple), hybridized oligos (yellow) and plasmids can be combined in different combinations to create targeting plasmids of varying complexity. C) For creating simpler constructs, entry clones which combine slots are used. D) Clone libraries of promoters, fluorescent proteins, linkers, and 3' UTRs provide a wealth of reagents for assembling new constructs. E-F) A variety of KanR vectors facilitates creating novel insert clones using efficient BsaI GG-based assembly strategies. E) A BsaI GG strategy can be used to create novel entry clones by amplifying the insert with oligonucleotides containing BsaI sites and performing a GG reaction into KanR vectors. F) BsaI and SapI sites in inserts can be removed using a BsaI GG strategy that lesions each site in the insert. G) Screen capture of a portion of the Excel-based 'GG plasmid builder' assembly tool which facilitates visualization of construct design options and testing of designs in silico. S, SapI sites; B, BsaI sites. Plasmids and DNA fragments are not drawn to scale.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red).