Figure 1. Subcellular Localization of MIG-14 and Wls (A) Expression of
mig-14::gfp (huIs71) in the posterior half of an early L1 larva (top panel). The boxed area shows the mesoblast cell M, which is magnified in the bottom panel. The outline of the M cell is indicated by arrowheads. Scale bar, 4 mm. (B) HeLa cells grown on glass coverslips were transfected with Wls-GFP and fixed to determine the intracellular localization of Wls-GFP. Bars, 20 um. Images are representative of at least three independent experiments. Dashed box is magnified and represented in the right panel. Arrows indicate plasma membrane localization of Wls. (C) HeLa cells grown on glass coverslips were transfected with Wls-GFP, fixed, and stained for endogenous Golgin-97, EEA1, Vps26, caveolin-1, or AP-2a. Scale bars, 10 um. Images are representative of at least three independent experiments. Dashed boxes are magnified and represented in the lower panels. White arrows indicate colocalization of Wls with EEA1, Vps26, or AP-2a.