Picture from Ghosh D et al. (2008) Genetics "Inhibition of transcription by the Caenorhabditis elegans germline protein ...."

GFP:PIE-1 localization in nuclei. Collapsed confocal Z-stacks through the P2 germline blastomere show accumulation of GFP:PIE-1 in nuclei. Wild-type PIE-1 accumulates in numerous fine nuclear foci. Note that PIE-1 also accumulates in larger cytoplasmic granules (P granules), which are visible around the nuclei in the micrographs.

Picture from Ghosh D et al. (2008) Genetics "Inhibition of transcription by the Caenorhabditis elegans germline protein ...."

Figure 5. GFP:PIE-1 localization in nuclei. Collapsed confocal Z-stacks through the P2 germline blastomere show accumulation of GFP:PIE-1 in nuclei. Wild-type PIE-1 accumulates in numerous fine nuclear foci. This localization is disrupted in PIE-1 mutants that do not suppress P-Ser5 efficiently (marked with asterisk). Reductions in CDK-7, CIT-1.1/1.2, and AMA-1 (RNA polymerase II) levels also affect this localization. Note that PIE-1 also accumulates in larger cytoplasmic granules (P granules), which are visible around the nuclei in the micrographs.

Picture from Ghosh-Roy A et al. (2012) Dev Cell "Kinesin-13 and tubulin posttranslational modifications regulate microtubule ...."

Figure S6, related to Figure 6. Reduced CCPP-6::GFP and increased TTLL-9::GFP expression in dlk-1(lf) mutantsConfocal projections of head neurons in functional CCPP6::GFP (A) and TTLL-9::GFP (B) reporters in wild type and in dlk1(ju476), imaged under identical conditions. CCPP-6::GFP (tzEx381) is expressed in several anterior neurons (arrows) (Kimura et al., 2010); this expression is significantly reduced in dlk-1(lf). Conversely, neuronal expression of a TTLL-9::GFP reporter (tzEx1610) is significantly increased in dlk-1(ju476). Arrows indicate TTLL-9::GFP puncta in anterior cilia. (C) Quantitation of cell body fluorescence intensity in anterior neurons, normalized to wild type. n=10 animals per genotype; statistics, Mann-Whitney test

Picture from Ghosh-Roy A et al. (2012) Dev Cell "Kinesin-13 and tubulin posttranslational modifications regulate microtubule ...."

(C) Quantitation of cell body fluorescence intensity in anterior neurons, normalized to wild type. n=0 animals per genotype; statistics, Mann-Whitney test

Picture from Ghosh S et al. (2016) MicroPubl Biol "ina-1 expression in the somatic gonad during late larval development."

ina-1 is expressed throughout uterine toroids. (A) At mid L4, expression is seen in uterine toroid 1 and 4 (ut1, ut4) and in the anterior uterine toroids 2 and 3 (ut2, ut3). In addition, ina-1::GFP is detected in the spermathecal-uterine junction and spermatheca. (B) At the L4 lethargus stage, bright expression is observed in uterine toroids 1 and 4, the spermathecal-uterine junction, and spermatheca. Dimmer expression is present in uterine toroid 2 and posterior toroid 3. Fluorescence is also detected in vulE. Anterior to the left, dorsal top. Scale bar 100 um. - Reviewed by Bhagwati Gupta.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.