Picture from Ghose P et al. (2013) PLoS Genet "Anoxia-reoxygenation regulates mitochondrial dynamics through the hypoxia ...."

Figure S4 STL-1 is broadly expressed in most tissues. (A) Schematic of stl-1 genomic DNA organization and associated transgenes for examining STL-1 expression and subcellular localization. Boxes indicate coding sequences within exons. The arrows indicate the start site of transcription. Brackets indicate regions that encode the indicated protein domains. The green line indicates the region of genomic DNA deleted in the tm1544 mutant. The green box indicates GFP sequences for the indicated reporter transgenes. (B-D) Fluorescence from Pstl-1::GFP in (B) body wall muscles, (C) intestinal epithelia, and (D) pharynx and head neurons. Bar, 5 mm.

Picture from Ghose P et al. (2013) PLoS Genet "Anoxia-reoxygenation regulates mitochondrial dynamics through the hypoxia ...."

GFP::DRP-1 was observed along ventral cord neurites of wild-type nematodes under conditions of normoxia. Bar, 5 mm.

Picture from Ghose P et al. (2013) PLoS Genet "Anoxia-reoxygenation regulates mitochondrial dynamics through the hypoxia ...."

STL-1 resides at mitochondria and is regulated by SKN-1. The fluorescence of (A,B) STL-1::GFP from a Pglr-1::STL-1::GFP transgene, and (C,D) TOM20::mCherry from a Pglr-1::TOM20::mCherry transgene, was observed in (A,C,E) command interneuron cell bodies, including PVC, and (B,D,F) ventral cord neurites of wild-type animals. (E,F) Merged images. The fluorescence of (G) STL-1::GFP from a Pstl-1 ::STL-1::GFP transgene was observed in the hypodermal epithelia of wild-type animals stained with (H) MitoTracker Red. (I) Merged image.

Picture from Ghose P et al. (2013) PLoS Genet "Anoxia-reoxygenation regulates mitochondrial dynamics through the hypoxia ...."

Figure 7. STL-1 resides at mitochondria and is regulated by SKN-1. The fluorescence of (A,B) STL-1::GFP from a Pglr-1::STL-1::GFP transgene, and (C,D) TOM20::mCherry from a Pglr-1::TOM20::mCherry transgene, was observed in (A,C,E) command interneuron cell bodies, including PVC, and (B,D,F) ventral cord neurites of wild-type animals. (E,F) Merged images. The fluorescence of (G) STL-1::GFP from a Pstl-1 ::STL-1::GFP transgene was observed in the hypodermal epithelia of wild-type animals stained with (H) MitoTracker Red. (I) Merged image. (J) Mean levels of stl-1 mRNA (relative to wild type) as measured by qRTPCR in the indicated genotypes and conditions. Red bars indicate normoxia, blue bars indicate anoxia, and gray bars indicate paraquat treatment. Bar, 5 mm.

Picture from Ghose P et al. (2013) PLoS Genet "Anoxia-reoxygenation regulates mitochondrial dynamics through the hypoxia ...."

Figure S1 Anoxia does not grossly alter the recruitment of mitochondrial dynamics machinery. The fluorescence from either (A-C) GFP::DRP-1 or (D-F) EAT-3::GFP was observed along ventral cord neurites of wild-type nematodes under conditions of (A,D) normoxia, (B,E) following 24 hours of anoxia, or (C,F) following 8 hours of reoxygenation after anoxic exposure. Bar, 5 mm.

Picture from Ghose P et al. (2013) PLoS Genet "Anoxia-reoxygenation regulates mitochondrial dynamics through the hypoxia ...."

EAT-3::GFP was observed along ventral cord neurites of wild-type nematodes under conditions of normoxia. Bar, 5 mm.

Picture from Ghose P et al. (2013) PLoS Genet "Anoxia-reoxygenation regulates mitochondrial dynamics through the hypoxia ...."

(B-D) Fluorescence from Pstl-1::GFP in (B) body wall muscles, (C) intestinal epithelia, and (D) pharynx and head neurons. Bar, 5 mm.

Picture from Claycomb JM et al. (2009) Cell "The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric ...."

CSR-1 (left, yellow) remain localized to P granules in the embryonic P cell lineage (dashed circles; DNA, center, blue).

Picture from Koh K et al. (2002) Development "Cell fates and fusion in the C. elegans vulval primordium are regulated by the ...."

Expression of egl-18::GFP. (A-C) Expression of pKK63 containing an 800 bp enhancer and a basal promoter. (A) L2 larva, in which all VPCs (P3.p-P8.p) show approximately equal levels of GFP expression (arrows). All six VC neurons show GFP expression, although not all are visible in this focal plane. Arrowhead indicate a VC neuron. (B) L3 larva shortly before VPC divisions, showing stronger GFP expression in P5.p-P7.p cells than in P4.p and P8.p. (C) L3 larva after the first VPC cell divisions. Daughters of P6.p show higher GFP expression than daughters of P5.p and P7.p. GFP expression is not detectable in P4.px and P8.px cells.

Picture from Inoue T et al. (2004) Cell "C. elegans LIN-18 is a Ryk ortholog and functions in parallel to LIN-17/Frizzled ...."

Corresponding Nomarski (bottom) and epifluorescence (top) images are shown. The anchor cell is indicated by large arrowheads. Identities of VPC descendants are indicated below. Scale bar = 20 μm.(B) Nuclear localized GFP under the control of the lin-17 promoter in P5.p and P7.p granddaughters. Arrows point to ventral cord neurons that also express lin-17::gfp. Although animals photographed in (B) display brighter expression in P5.p and P7.p compared to P6.p, many individuals express lin-17::gfp in P6.p granddaughters also.(E) lin-17::gfp in VPCs, P6.p, and P7.p. P5.p also expresses lin-17::gfp and lin-18::gfp (not shown). Downward arrows point to ventral cord neurons expressing lin-17::gfp.