Picture from Gerson-Gurwitz A et al. (2016) Cell "A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to ...."

Figure S2. The csr-1(tm892) Mutant, GFP::KBP-4 Localization in csr-1(RNAi), the gfp::csr-1 Transgene Insertion, and GFP::CSR-1 Localizationin the Absence of Endogenous CSR-1, Related to Figure 2(A) The csr-1(tm892) mutant. The 400 bp deletion in tm892 removes the indicated intronic and exonic sequences and introduces a frameshift mutation after residue number 44 of CSR-1b (which is 847 aa long), resulting in encoding of 13 additional out-of-frame amino acids followed by a stop codon.(B) Line scan analysis of GFP::KBP-4 in control and csr-1(RNAi). KBP-4 is a subunit of the microtubule-binding NDC-80 complex. n is the number of chromosomes analyzed. For representative images, see Figure 2F.(C) Schematic of the gfp::csr-1 transgene inserted in single copy at a Mos transposon site on Chr II. The coding sequence for GFP was inserted at a position that results in translation of GFP after aa 5 (encoded by exon 1) of CSR-1b and after aa 168 (encoded by exon 2) of CSR-1a. The transgene was also recoded for RNAi resistance as described in Figure S3A.(D) The gfp::csr-1 transgene rescues the embryonic lethality and sterility of csr-1(tm892). Embryo viability and brood size was measured for the indicatedconditions. For csr-1(tm892), first generation homozygous worms derived from balanced heterozygous mothers were analyzed. Error bars are the SD.(E) Localization of GFP::CSR-1 in early embryos (left panel set) and in the larval and adult germline (right panel set). P-granule localization is highlighted by green arrows in the embryo posterior (left panels) and in the perinuclear region in the germline (green arrows). The blowup of the L4 larva germline image (yellow box) shows the clustered perinuclear signal. Blowups of two oocyte nuclei in the adult germline highlight the GFP::CSR-1 signal in the nuclear periphery and absence of detectable chromosome enrichment. Scale bars 10 um in all images except magnified region of L4 larva germline, where scale bar is 5 um.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red).

Picture from Liu H et al. (2006) Development "Direct regulation of egl-1 and of programmed cell death by the Hox protein MAB-5 ...."

CEH-20 is expressed in P11.aaap. DIC (A) and epifluorescence (B) images. (A,B) The posterior ventral nerve cord of a late L2 stage animal carrying a rescuing Pceh-20ceh-20:cfp fusion gene.