FIG. 4. Expression pattern of the CePAK-GFP reporter without NLS. pCPK1.81 (Fig. 2) was introduced into the
dpy-20 strain and the transformed animals were observed. Each pair of panels show the fluorescent image (A, C, E, G and I) and the Nomarski image (B, D, F, H and J) viewed at the same focal plane. A, B. Expression in the distal tip cell (DTC, arrowheads). The gonad (arrow in B) follows the DTC. Germ-line nuclei in the gonad are evident in B. C, D. Expression in the spermatheca (arrows). E, F. Expression in the uterus. Expression in one of the embryos is also seen (arrowhead). The vulva is shown by the arrow in F. G-J. Expression in the embryo. G, H and I, J show different focal planes of the same embryo. G, H. Close to the surface of the embryo. I, J. Along the midline of the embryo. Bar: 20um.