Figure 1. Quantification of C. elegans' cell and tissue volumes: a) Workflow of our approach. b) On the left: tissue volumes determined with the two Blender plugins '3D print' or 'Mesh Calculator'. For the gonad and reproductive system, the volume comprising the rachis and germ cells filling it in a lighter shade to distinguish them from somatic tissue. On the right: visualization of the analyzed tissues. c) Top: relative contribution of tissues to the final volume. The extracellular cuticle was excluded from this analysis. Bottom: same analysis but including rachis and non-somatic germ cells. d) Top: relative contribution of tissues to the number of nuclei, the number of diploid genomes. Bottom: same analysis but including the non-somatic germ cells. e) Cell volume, grouped by different tissues, displayed in a linear y- axis. Rachis and non-somatic germ cells were excluded from the analysis. f) Cell volume normalized per diploid genome, grouped by different tissues, displayed in a
log10 y-axis. Rachis and non-somatic germ cells were excluded from the analysis. g) Distribution of cell volumes (
log10 y-axis). h) Distribution of cell volumes per diploid genomes (
log10 y-axis). For details, raw data, and calculations, please see Extended Data.