Picture from Hobert O et al. (1999) Development "The Caenorhabditis elegans lim-6 LIM homeobox gene regulates neurite outgrowth ...."

Figure 2. lim-6 reporter gene expression in neural and non-neural tissues. (A) Schematic drawing of the position and axonal morphology of all lim-6-expressing neurons. (B-F) Expression of the lim-6 reporter gene constructs in late larval stages. (B) Expression of lim-6prom::GFP in ASEL and the excretory gland (EG) cells. Note that there are two EG cell bodies which send out processes which fuse at two different places to yield a binucleate cell. (C) Expression of lim-6int3::GFP in AVL and RIS. (D) Expression of lim-6r::GFP in the head and (E) in the tail. (F) Expression of lim-6prom::GFP in the uterus of an L4 stage animal. (G) DIC photomicrograph of the same animal as shown in F. Note that in B, C and F the GFP reporter gene is localized throughout the cell presumably due to the absence of a nuclear localization sequence in the only partial LIM-6 sequences. In D and E the presence all LIM-6 protein codings sequences reveals its nuclear localization. The sites of expression of the respective reporter gene constructs are summarized in Table 1.

Picture from Mallick, Rishab et al. (2022) MicroPubl Biol

Figure 1. Overview of the data extraction process and key results(A) Using database publication IDs as input, the sentences from the corresponding papers are extracted using wbtools or TextPresso, and then processed by the hybrid system where all mutation mentions are extracted. Additional information relevant for curation is then extracted using bag-of-words. The gene and mutation matches are verified by validating that the reference base/codon is possible in at least one transcript in the gene. The final matches and additional data (eg strain name) are then presented in a structured output for validation by curators before being used to update the database. (B) Metrics on sentence classification with mutation mention tested on IDP4+ corpus.

Picture from Kubota Y et al. (2006) Development "The conserved oligomeric Golgi complex acts in organ morphogenesis via ...."

Figure 5. Expression of COGC-3. (A-C) Immunolocalization of COGC-3 in a cross-section of a wild-type L4 larva. The section was stained with anti-COGC-3 (red), fluorescein phalloidin (green) and DAPI (blue). (A) Merged image. (B) Anti-COGC-3 image. (C) Schematic drawing of the positions of tissues. Arrowhead indicates the perinuclear localization of COGC-3 in a muscle cell. The muscle cells contain actin filaments stained by fluorescein phalloidin apically and the nuclei basally. (D) Localization of COGC-3 in a cogc-3(k181) L4 larva. No COGC-3 signal was detected. Dorsal is towards the top. Scale bar: 12.5 μm. (E-J) Whole-mount immunohistochemistry with COGC-3 antibody. Antibody staining patterns (EG,I) and corresponding schematic drawings (F,H,J) of wild-type L4 larvae. (E,F) Pharynx, (G,H) vulva and (I,J) seam cell. Dorsal is towards the top. Anterior is leftwards. Scale bar: 25 μm.

Picture from Lyssenko NN et al. (2008) BMC Dev Biol "An unexpectedly high degree of specialization and a widespread involvement in ...."

Figure 4. tat-2 reporter expression pattern. The 5' end of the tat-2 locus, locations of the deleted regions in tm1634 and tm1773 mutants (bars) and structure of the tat-2 expression cassette (A). Expression of the tat-2 reporter in the lcIs982.4.2 line in embryos (B), in the intestine of L2 larvae (C) and in gland cells of the pharynx, the pharyngeal-intestinal valve, the excretory gland cell and the intestine of a young adult (D). GFP fluorescence in the lcEx982.2.4.5 line in the excretory pore and excretory canal cells (E). Reporter signal outlining the vulF cells in early lcIs982.4.2 L4 larvae (the third frame in the series shows an overlay of the pseudocolored UV image onto the bright light image, both enlarged with re-sampling using Photoshop; dashes extend along the contact surface between the two tissues) (F). tat-2 reporter expression during spermatogenesis (G) and in adult spermatheca (H) of lcIs982.4.2 animals. Abbreviations: eg ' excretory gland cell; epc ' excretory pore cell; g 'pharyngeal gland cell; ga 'gonad arm; ph ' pharynx; pv ' pharyngeal-intestinal valve; oo ' oocyte; r ' rectum; sp ' spermatheca.