Picture from Frank DJ et al. (1998) J Cell Biol "ncl-1 is required for the regulation of cell size and ribosomal RNA synthesis in ...."

Figure 7. Localization of NCL-1 protein. (a and b) Immunofluorescence micrographs of wild-type embryos stained for NCL-1 protein with mAb D3C2 (a) and with DAPI to visualize nuclei (b). Intense staining is seen in the two-cell embryo (left), but is diminished by the 28-cell stage (right). (c and d) Immunofluorescence micrographs of two different wild-type 300-cell embryos stained with either mAb D3C2 to visualize NCL-1 protein (c) or with mAb K121 (d) to visualize nucleoli. At this stage, NCL-1 protein appears to be present in all cells except for those of the gut which have prominent nucleoli as detected by mAb K121. (e) Immunofluorescence micrograph of a wild-type gonad stained with mAb D3C2. Staining is most intense in the most mature oocyte (left). The large and small brackets indicate the most mature and second most mature oocytes, respectively. Bars, 10 um.