Picture from Dammermann A et al. (2009) Genes Dev "The hydrolethalus syndrome protein HYLS-1 links core centriole structure to ...."

(E) HYLS-1 localizes to the base of the cilium. L1 larva expressing CHE-11:GFP stained for DNA, tubulin and HYLS-1. Scalebars are 50μm (C, D), 5μm (E).

Picture from Alexander M et al. (2009) Development "An UNC-40 pathway directs postsynaptic membrane extension in Caenorhabditis ...."

(E-E'') UNC-73B::CFP specifically expressed in muscles in the background of the trIs34 integrated transgene that expresses UNC-40::YFP specifically in muscles. The localization of fusion proteins to the muscle arm termini is indicated with a yellow arrowhead. Scale bars: 50 μm in A-C; 10 μm in E-E''.

Picture from Amin NM et al. (2010) Development "The FoxF/FoxC factor LET-381 directly regulates both cell fate specification ...."

(B-D) Representative images of live embryos (B,C) and fixed late L1 larva (D) expressing let-381::gfp. (E-N) The left side of three L1 larvae double-labeled with LET-381::GFP (E,I), anti-FOZI-1 antibody (F,J) and DAPI (H) at the 14-M (E-H) and 16-M stage (I-K). (G,K) Merged images of E and F and of I and J, respectively. (L-N) An L1 larva double-labeled with anti-LET-381 (L) and anti-FOZI-1 antibodies (M) at the 16-M stage. (N) A merged image of L and M. (O) Summary of LET-381 expression in the M lineage, with LET-381-positive cells in green circles and CEH-34-positive cells outlined in black.

Picture from Pasierbek P et al. (2001) Genes Dev "A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome ...."

COH-2 is localized in embryonic nuclei (B) and forms foci from the mitotic up to the pachytene zone of the gonad (E, I). In pachytene nuclei the foci are exclusively peripheral (I). Bars: 10 μm in E.

Picture from Oka T et al. (2001) J Biol Chem "Four subunit a isoforms of Caenorhabditis elegans vacuolar H+-ATPase. ...."

Transgenic worms carrying the vha-6::GFP plasmid (pHJ-V6P03) were fixed with 4% paraformaldehyde in phosphate-buffered saline. Fluorescence confocal (E, G) and merged images with phase contrast (F, H) were acquired. E-H, L1 (E and F) and adult (G and H) worms harboring the vha-6::GFP plasmid (pHJ-V6P03). The vha-6::GFP fusion gene was expressed exclusively in intestinal cells (E andG, arrowheads). In, intestine. Scale bars, 50 μm.

Picture from Pasierbek P et al. (2001) Genes Dev "A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome ...."

Figure 6. Immunostaining of HIM-3 (A,B,E left: green) and REC-8 (C,D,E right: red) of spread nuclei at leptotene/zygotene (A,C) and pachytene (B,D,E). Note the parallel association of forming axial elements (presynaptic alignment) in A. Both proteins delineate the six synaptonemal complexes (SCs) at pachytene (B,D) and show nearly perfect colocalization by double immunostaining (E, center). Counterstaining of chromatin by DAPI (blue). Bar: 10 μm.

Picture from Wang L et al. (2017) PLoS Genet "Two Paralogous Tetraspanins TSP-12 and TSP-14 Function with the ADAM10 ...."

(A-P) Confocal fluorescent images (A, C, E, G, I, K, M,O) and corresponding DIC images (B, D, F, H, J, L, N, P) of transgenic worms expressing endogenous GFP::TSP-12 (A, C, E, G) or over-expressed (OE) TSP-12::GFP (I, K, M, O) in the early embryo (A), L4 developingvulva (C), late L3 hypodermis (E), young adult gonad (G), L3 larva head (I), L4 intestine (K: basolateral focal plane of the intestine and the arrow points to the lateral membrane, M: middle focal plane of the intestine andthe arrow points to the apical lumen of the intestine), and L3 neurons (O).

Picture from Fukuto HS et al. (2004) Neuron "G protein-coupled receptor kinase function is essential for chemosensation in ...."

(C and D) A Ce-grk-2::gfp transcriptional reporter is expressed in neurons in the body (C) and head (D). Broad expression in the nervous system and the vulval muscles is observed. Scale bar equals 100 μm in (C) and 25 μm in (D).(E and F) Ce-GRK-2 overexpressed using its own promoter can be detected in the nervous system with anti-mammalian GRK2/3 antibody. The entire animal (E) or just the head of the animal (F) are shown. Scale bar equals 100 μm in (E) and 25 μm in (F). No overt localization to sensory neuron cilia was observed, although overexpression of Ce-GRK-2 may mask or interfere with normal subcellular localization.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(E) Localization of GLA-3B in adult hermaphrodite gonads. Gonads were dissected from wild-type and gla-3(op212) worms 24 h after the L4/adult molt and were stained with the anti-GLA-3B-specific antibody 1297 and DAPI. Scale bar in C and E, 8 μm.

Picture from Ruaud AF et al. (2007) Development "The P-type ATPase CATP-1 is a novel regulator of C. elegans developmental timing ...."

(D-G) catp-1 expression profile. Detection of GFP fluorescence by confocal (D) and epifluorescence microscopy (E) in transgenic larvae expressing Pcatp-1(C. briggsae)::GFP. catp-1 is expressed in the hyp (white arrowheads) and Pn.p cells of the epidermis (D) and in the duct cell of the excretory system (E-G). No GFP expression was detected in the lateral cells of the epidermis (seam cells: black arrowheads). In (G), the GFP image (E) of the duct cell was overlayed on a Nomarski picture of the same field (F) where the excretory duct is visible (black arrow). Scale bars: 20 μm (D); 5μm (E-G).