G, H, PNG-1::GFP expressed from a pan-epithelial or pan-neuronal promoter shows cytoplasmic localization. Epithelial seam cells (G) and a tail neuron (H) are shown. Scale bars, 10 um.
Expression pattern of T22A3.3 (H) as determined by promoter-GFP reporter constructs. (H) Expression of T22A3.3 is seen in a set of neurons in the head, including AWB.
COH-1 is present in the nuclei of embryos (A) and of the distal tip cell (D). It also localizes to chromosomes in pachytene (G) to diakinesis (H). Bars: 5 μm in A, 5 μm in H.
(A) and (C): DIC images of the embryos shown in (B) and (D). E-H. At postembryonic stages, epg-11::gfp is expressed in pharyngeal cells (E,F, arrows) and vulval cells (G,H, arrow). (E) and (G): DIC images of the animals shown in (F) and (H).
G-H, an integrated functional Pnlf-1::NFL-1::GFP transgene was expressed in the cholinergic motor neurons (labeled by a Psto-6::wCherry marker), but not in the GABAergic motor neurons (labeled by a Punc-25::wCherry marker). Scale bars, 5 um (A-D), 10 um (G, H).
Expression of VPS-18. (A) VPS-18::GFP expression in embryo (a and b), larva (c and d), adult coelomocyte (e and f), and germline (g and h) as well as the dissected-out germline (i and j). Arrows in (h and j) indicate germline sheath cells. The scale bars represent 20 um (a-d, i, and j), 5 um (e and f), and 10 um (g and h).
(H-J) Immunostainings of a GFP-SAS-1 embryo for GFP (yellow) and IFA (magenta) (I), as well as of a wild type (H) or sas-1(t1476) (J) embryo stained for SAS-1 (yellow) and IFA (magenta). DNA is in cyan in all panels.