Picture from Wang Y et al. (2006) Proc Natl Acad Sci U S A "The C2H2 zinc-finger protein SYD-9 is a putative posttranscriptional regulator ...."

(A and B) C. elegans carrying a functional SYD-9::GFP construct stained with antibodies against GFP. (A) Larvae showing strong GFP expression in neurons of the ventral nerve cord, pharynx, and tail ganglia. Weak muscle expression is out of the focusing plane. (B) A ventral view of adults showing GFP in vulval and uterinal muscle nuclei. (C-E) Double staining with GFP and DAPI in hpIs6 animals carrying only the SYD-9B::GFP isoform. SYD-9B::GFP signal was predominantly localized at the interchromatin region (arrows). (Scale bars: A, 20 um; B, 3 um; C-E 1 um.)

Picture from Wang Y et al. (2006) Proc Natl Acad Sci U S A "The C2H2 zinc-finger protein SYD-9 is a putative posttranscriptional regulator ...."

(F-H) Staining of WT animals with SYD-9 antibody N (F and G) and SYD-9 antibody C (H). SYD-9 is expressed in the nuclei of body wall muscles (arrow) and ventral cord motoneurons (arrowhead). At higher magnification, SYD-9 expression shows a distinct subnuclear pattern similar to that of SYD-9::GFP; in particular, antibody C shows a speckle-like pattern (G and H). Antibody N staining in the retrovesicular ganglion neurons (G) and antibody C staining in the nerve ring ganglion neurons (H) are shown. (I and J) Western blot analysis of mixed-stage C. elegans lysates by using antibody N (I) and antibody C (J). Tubulin is the loading control for lysate. (Scale bars: A, 20 um; B and F, 3 um; C-E, G, and H, 1 um.)

Picture from Wang Y et al. (2006) Proc Natl Acad Sci U S A "The C2H2 zinc-finger protein SYD-9 is a putative posttranscriptional regulator ...."

SYD-9 proteins are predominantly expressed in nuclei of neuron and muscle cells. (A and B) C. elegans carrying a functional SYD-9::GFP construct stained with antibodies against GFP. (A) Larvae showing strong GFP expression in neurons of the ventral nerve cord, pharynx, and tail ganglia. Weak muscle expression is out of the focusing plane. (B) A ventral view of adults showing GFP in vulval and uterinal muscle nuclei. (C-E) Double staining with GFP and DAPI in hpIs6 animals carrying only the SYD-9B::GFP isoform. SYD-9B::GFP signal was predominantly localized at the interchromatin region (arrows). (F-H) Staining of WT animals with SYD-9 antibody N (F and G) and SYD-9 antibody C (H). SYD-9 is expressed in the nuclei of body wall muscles (arrow) and ventral cord motoneu- rons (arrowhead). At higher magnification, SYD-9 expression shows a distinct subnuclear pattern similar to that of SYD-9::GFP; in particular, antibody C shows a speckle-like pattern (G and H). Antibody N staining in the retrovesicular gan- glion neurons (G) and antibody C staining in the nerve ring ganglion neurons (H) are shown. (I and J) Western blot analysis of mixed-stage C. elegans lysates by using antibody N (I) and antibody C (J). Tubulin is the loading control for lysate. (Scale bars: A, 20 um; B and F, 3 um; C-E, G, and H, 1 um.)

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.

Picture from Alper S et al. (2002) Development "The zinc finger protein REF-2 functions with the Hox genes to inhibit cell fusion ...."

Figure 7. ref-2 is expressed in the P cell lineage in wild-type hermaphrodites. Staged population of L1 larvae were stained with an anti-REF-2 antiserum (red in A,D,G,J) and the MH27 monoclonal antibody (green in B,E,H,K) as described in the Materials and Methods. Merged images are shown in C,F,I,L. REF-2 is initially detected in the 12 P cells just prior to their migration into the ventral cord (A-C; ventral view) and remains on as the P cells migrate (D-F; ventral view). In this picture, P1 and P2 have migrated; the other P cells will migrate soon. REF-2 is then present in both daughters of all P cells following division (G-I; ventrolateral view). REF-2 then disappears in the Pn.a cells and their progeny. REF-2 also disappears in the Pn.p cells, although it does so at different rates. REF-2 remains on longest in the six unfused Pn.p cells P(3-8).p (J-L, lateral view). White arrowheads indicate P1/2, P3/4,..P9,10 in A, P1 and P2 (which have migrated) and P3/4, P5/6, P7/8, P9/10 in D, and P(3-8).p in J.

Picture from Dammermann A et al. (2008) J Cell Biol "SAS-4 is recruited to a dynamic structure in newly forming centrioles that is ...."

(A) High-resolution images of GFP:SAS-6 recruited to RFP-labeled sperm centrioles in embryos generated using the mating scheme in Fig. 1 A. Times (in seconds relative to cytokinesis onset) correspond to meiosis (-1,470 to -1,370 s), early (-1,180 to -1,156 s) and mid (-1,005 to -972 s) S phase, and late prophase (-281 to -267 s). (B) Normalized individual measurements of the integrated GFP:SAS-6 intensity coincident with sperm centriolar RFP signal.

Picture from Cameron S et al. (2002) Development "PAG-3, a Zn-finger transcription factor, determines neuroblast fate in C. ...."

Figure 5. Expression pattern of PAG-3 during postembryonic development of the ventral nerve cord. (A,B) PAG-3 antibody staining of wild-type L1/L2 larvae. In all panels, anterior is leftwards and ventral is downwards. (C,D) DAPI co-staining of animals in A,B. In the postembryonic ventral cord, anterior lineages generally divide earlier than posterior lineages (Sulston and Horvitz, 1977). (A,C) In this animal the P6.aa, P7.aa and P8.aa neuroblasts have divided, while P10.aa and P6.ap are in the process of mitosis. The P9.aa and P11.aa neuroblasts have not yet divided. P12.aa and P12.ap are out of the focal plane in the preanal ganglion. PAG-3 protein was detected in the Pn.aa, but not in the Pn.ap, neuroblasts. By examining the staining of many animals at various stages of development, we could trace PAG-3 protein continuously from the Pn.aa neuroblasts to all three neurons generated by them. In A,C, the Pn.aaa neuroblasts were larger and appeared brighter than their posterior sister cells, the Pn.aap cells, which terminally differentiate (P3-P8.aap) or undergo programmed cell death (P1-P2.aap, P9-P12.aap). (B,D) PAG-3 protein in each of the three terminally differentiating neurons generated by the Pn.aa neuroblasts. (E) Diagram of P cell lineage with cells containing PAG-3 protein indicated by green lines. Pn.aa neuroblasts did not contain detectable PAG-3 when they were generated, but became positive a short time later.

Picture from Cameron S et al. (2002) Development "PAG-3, a Zn-finger transcription factor, determines neuroblast fate in C. ...."

Figure 6. Identification of PAG-3-staining ventral cord neurons in adult animals. Otherwise wild-type adult C. elegans carrying an integrated Punc-4lacZ transgene were co-stained with PAG-3 antiserum and monoclonal antibody directed against beta-galactosidase. DAPI was used to identify nuclei. (A-D) The posterior ventral cord; anterior is leftwards and ventral is downwards. VA11 and VA12 are marked with open triangles. (E-H) The retrovesicular ganglion and anterior ventral cord; ventral view, anterior is leftwards. AVFR and AVFL are marked with filled triangles. (A) DAPI, (B) beta-galactosidase expression in VA11 and VA12 (Miller and Niemeyer, 1995). (C) PAG-3 staining. (D) Merged image. (E) DAPI staining of nuclei in the retrovesicular ganglion. (F) beta-galactosidase expression in five cells in the retrovesicular ganglion. Punc-4lacZ is expressed in the AVF interneurons, the VA motoneurons, and additional cells in the retrovesicular ganglion (Miller and Niemeyer, 1995). (G) PAG-3 staining. By several criteria (see text), the two anterior-most PAG-3 expressing cells in the retrovesicular ganglion are the right and left AVF interneurons. The two more posterior PAG-3-positive cells in the retrovesicular ganglion are the embryonically generated right and left RIG interneurons; they are marked with asterisks. (H) Merged image.