Top panel: fluorescence and bright field images of mCherry expressing embryos; middle and bottom panels: fluorescence and bright field images of adults (head to the left). Plin-44::mCherry is mainly expressed in the tail during the embryonic and adult stages.
Top panel: fluorescence and bright field images of mCherry expressing embryos; middle and bottom panels: fluorescence and bright field images of adults (head to the left). Pegl-20::mCherry is mainly expressed in the tail during the embryonic and adult stages.
Top panel: fluorescence and bright field images of mCherry expressing embryos; middle and bottom panels: fluorescence and bright field images of adults (head to the left). Pmom-2::mCherry is robustly expressed in the tail and weakly expressed in the vulva and body wall muscles.
Top panel: fluorescence and bright field images of mCherry expressing embryos; middle and bottom panels: fluorescence and bright field images of adults (head to the left). Pcwn- 1::mCherry is robustly expressed in the tail and weakly expressed in the vulva and body wall muscles.
(G to I) Expression pattern of the Venus reporter driven by snet-1p. Bright-field image of (G) is shown as an inset. (H) shows a bright-field image of the head region observed in (I). Question marks indicate an unidentified pair of lateral neurons.
Supplementary figure 1. Expression of LET-767::GFP. A, in embryos. B, bright field image of A. C, strong expression, detected in larval intestine. D, bright field image of C. E, weaker expression, detected in pharyngeal region (arrowhead) and in the hypodermis. F, bright field image of E. G, subcellular localization of LET-767::GFP in intestinal cells. H, accumulation of LET-767::GFP at the apical surface of intestinal cells. Scale bars: A, B, E-F - 20 um, C, D - 50 um, G, H - 10 um.
Tissue-specific expression pattern of cenadc1. Expression of GFP was driven by the cenadc1 gene promoter in stably transformed transgenic C. elegans. The inset A1 is the bright field image (low magnification) of the animal for A. D. CFP expression driven by the cenadc1 promoter. The inset D1 is the bright field image of the same worm for D.
Worm SREBP (Y47D38.7) is expressed in the intestine. Transgenic worms expressing an SREBP::GFP translational fusion were examined by bright-field and GFP fluorescence microscopy.
(A) lpd-3 is expressed in the intestine. Transgenic worms expressing an lpd-3::GFP translational fusion were examined by bright-field and GFP fluorescence microscopy.