Picture from Fei YJ et al. (2003) J Biol Chem "Structural and functional characteristics of two sodium-coupled ...."

Tissue-specific expression pattern of cenadc1. Expression of GFP was driven by the cenadc1 gene promoter in stably transformed transgenic C. elegans. The inset A1 is the bright field image (low magnification) of the animal for A. D. CFP expression driven by the cenadc1 promoter. The inset D1 is the bright field image of the same worm for D.

Picture from Fei YJ et al. (2003) J Biol Chem "Structural and functional characteristics of two sodium-coupled ...."

Developmental stage-specific expression of ceNaDC2. Following RT-PCR, 10 μl of the products were separated in a 1.2% (w/v) agarose gel to show the size and intensity of the cenadc gene-specific and the 18S rRNA-specific fragments. A 1.0-kb DNA marker (Invitrogen) was used as a molecular mass standard. The upper bands (~ 850 bp long) were derived from the cenadc2 transcripts; the lower bands (~ 480 bp) were amplified from 18S rRNA and served as an internal control. The RNA samples that served as templates were prepared from different developmental stages of C. elegans: embryos (Emb), early larva stage (L1&2), late larva stage (L3&4), and adults. In addition, RNA prepared from a mixture of C. elegans at different developmental stages was also used (Mix).

Picture from Fei YJ et al. (2003) J Biol Chem "Structural and functional characteristics of two sodium-coupled ...."

Figure 4. Developmental stage-specific expression of ceNaDC1 and ceNaDC2. Following RT-PCR, 10 μl of the products were separated in a 1.2% (w/v) agarose gel to show the size and intensity of the cenadc gene-specific and the 18S rRNA-specific fragments. A 1.0-kb DNA marker (Invitrogen) was used as a molecular mass standard. The upper bands (~ 850 bp long) were derived from the cenadc1 (upper panel) and cenadc2 (lower panel) transcripts; the lower bands (~ 480 bp) were amplified from 18S rRNA and served as an internal control. The RNA samples that served as templates were prepared from different developmental stages of C. elegans: embryos (Emb), early larva stage (L1&2), late larva stage (L3&4), and adults. In addition, RNA prepared from a mixture of C. elegans at different developmental stages was also used (Mix).

Picture from Fei YJ et al. (2003) J Biol Chem "Structural and functional characteristics of two sodium-coupled ...."

Figure 5. Tissue-specific expression pattern of cenadc1 and cenadc2 genes. Expression of GFP was driven by the cenadc1 (A) and cenadc2 (B) gene promoters in stably transformed transgenic C. elegans. The insets A1 and B1 are the bright field images (low magnification) of the animals for A and B, respectively.C, (YFP expression driven by the cenadc2 promoter) and D (CFP expression driven by the cenadc1 promoter) show the expression pattern of YFP and CFP in the same animal. The inset C1/D1 is the bright field image of the same worm for both C and D.

Picture from Fei YJ et al. (2003) J Biol Chem "Structural and functional characteristics of two sodium-coupled ...."

Tissue-specific expression pattern of cenadc2. Expression of GFP was driven by the cenadc2 gene promoter in stably transformed transgenic C. elegans. The inset B1 is the bright field image (low magnification) of the animals for B. C, (YFP expression driven by the cenadc2 promoter) shows the expression pattern of YFP. The inset C1 is the bright field image of the same worm for C.

Picture from Fei YJ et al. (2003) J Biol Chem "Structural and functional characteristics of two sodium-coupled ...."

Developmental stage-specific expression of ceNaDC1. Following RT-PCR, 10 μl of the products were separated in a 1.2% (w/v) agarose gel to show the size and intensity of the cenadc gene-specific and the 18S rRNA-specific fragments. A 1.0-kb DNA marker (Invitrogen) was used as a molecular mass standard. The upper bands (~ 850 bp long) were derived from the cenadc1 transcripts; the lower bands (~ 480 bp) were amplified from 18S rRNA and served as an internal control. The RNA samples that served as templates were prepared from different developmental stages of C. elegans: embryos (Emb), early larva stage (L1&2), late larva stage (L3&4), and adults. In addition, RNA prepared from a mixture of C. elegans at different developmental stages was also used (Mix).

Picture from Fei YJ et al. (2000) J Biol Chem "A novel H(+)-coupled oligopeptide transporter (OPT3) from Caenorhabditis ...."

Figure 8. GFP-expression pattern driven by the opt3 gene promoter in stably transformed transgenic C. elegans. A shows a double-exposed image of AVA cell body in green (fluorescein isothiocyanate filter) indicated by an arrow, superimposed with that of the surrounding amphid neurons (ASK, ADL, ASI, ASH, and ASJ) and their processes (arrowhead) stained with DiI (Texas Red filter) in the vicinity. The intestines are also shown because of auto-fluorescent granules. B shows both AVAR and AVAL neuron cell bodies (arrows) and the processes (arrowheads). The camera lucid drawing on the right depicts the positions of the neurons and processes for clarity.

Picture from Fei YJ et al. (2004) Biochem J "Relevance of NAC-2, an Na+-coupled citrate transporter, to life span, body size ...."

Figure 3. Tissue expression pattern of nac-2 gene in C. elegans. Expression of GFP was driven by the nac-2 gene promoter in the transformed transgenic C. elegans. A GFP image of a representative young adult worm is shown and the inset is the bright-field image.