(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.
TBB-4 fusion protein localizes to sensory cilia. G-H show the localization of the indicated tubulin::YFP fusion protein expressed under the control of the TBB-4 promoter. Dotted lines indicate the outline of the body and the fan, the cuticular layer in which the rays are embedded. G shows nose cilia, while the other panel shows a view of the male tail. All images are flattened stacks derived from multiple confocal sections spanning planes containing nose or ray cilia. Solid arrowheads indicate examples of the localization of tubulin fusion proteins to cilia.
Expression pattern of T22A3.3 (H) as determined by promoter-GFP reporter constructs. (H) Expression of T22A3.3 is seen in a set of neurons in the head, including AWB.
(F-H) DAF-16 becomes more cytoplasmic with age, and nuclear localization of PQM-1::GFP decreases significantly with age. Data are represented as mean +/- SEM (G and H).
(A) and (C): DIC images of the embryos shown in (B) and (D). E-H. At postembryonic stages, epg-11::gfp is expressed in pharyngeal cells (E,F, arrows) and vulval cells (G,H, arrow). (E) and (G): DIC images of the animals shown in (F) and (H).