Figure 3
lsy-27 is expressed and acts during the initiation but not during the maintenance phase of left/right asymmetry control. (A) Expression pattern of
lsy-27fosmid::yfp (shown in Figure 2A) at different embryonic stages. The embryos appear slightly deformed as they are squished together in the gonad of an adult animal. The white arrow indicates the ASEL neuron shortly after birth based on colocalization with a bilateral ASE-specific reporter otIs232(
che-1::mCherry) (not shown). The lsy- 27transl::gfp (shown in Figure 2A) shows a similar expression pattern except that, due to its failure to be expressed in the germline, we see only the onset of expression when zygotic gene expression starts in the early embryo. Bar, 10 mm. (B) Temperature-shift experiments with
ot108; ntIs1 animals indicate that
lsy-27 activity is required only during embryogenesis, but not during postembryonic stages. Animals were cultured for at least three generations at either 15 or 25. Animals were analyzed by isolating two- to four-cell embryos and temperature shifts were performed at various developmental stages. All animals were scored as 3-day-old adults.