Testing the role of
cdr-6 in the C. elegans zinc response. A) Results from linkage mapping analysis using expression of
cdr-6 as a quantitative trait. Genomic position (x-axis) is plotted against the logarithm of the odds (LOD) score (y-axis) for 13,003 genomic markers. Each significant QTL is indicated by a red triangle at the peak marker, and a blue rectangle shows the 95% confidence interval around the peak marker. The percentage of the total variance in the RIAIL population that can be explained by each QTL is shown above the QTL. B) For each QTL, the expression of
cdr-6 (y-axis) in RIAILs split by genotype at the marker with the maximum LOD score (x-axis) are plotted as Tukey box plots. Each point corresponds to the relative expression of a unique recombinant strain. Strains with the N2 allele are colored orange, and strains with the CB4856 allele are colored blue. C) Gene model for
cdr-6 is shown. Exons are represented by light purple rectangles and introns are represented by connecting lines. Location of the CRISPR-Cas9 deletion is shown with the grey box below the gene model. D) Strain genotypes are shown as colored rectangles (N2: orange, CB4856: blue) in detail for chromosome V (left) and in general for the rest of the chromosomes (right). The solid vertical line represents the peak marker of the zinc-response QTL, and the dashed vertical lines represent the confidence interval. Grey triangles represent
cdr-6 deletions. E) Relative animal optical density in zinc (median.EXT, x-axis) is plotted as Tukey box plots against strain (see C, y-axis). Statistical significance of each strain compared to its parental strain (ECA1330 and ECA1332 to N2 and ECA1333 and ECA1334 to CB4856) is shown above each strain and colored by the parent strain it was tested against (ns = non-significant, p-value > 0.05; * = significant, p-value < 0.05).