Figure 4. PAR-3 Distribution in 1-Cell Embryos. Double exposure of 1-cell embryos stained for PAR-3 using an FITC- labeled secondary antibody and costained with DAPI. (A-C) Wild-type embryos. (D-F) par mutant embryos. (A) Wild-type 1-cell embryo prior to pronuclear migration. PAR-3 can be seen at the anterior periphery.(B) Anaphase stage wild type. PAR-3 is at the anterior periphery. (C) Telophase stage wild type. PAR-3 staining is associated with the cleavage furrow. (D) A 1-cell parm7
(b274) embryo. PAR-3 localization is the same as in wild type. (E) A pwZ
(lw32) zygote. PAR-3 is found at the cell periphery, in a graded distribution with a higher concentration at the anterior, tapering off to a barely detectable amount at the posterior. (F) No signal can be detected at the periphery of
par-3(
it77) embryos, and the cytoplasmic staining is considerably reduced.All embryos are oriented with anterior at the left. The scale bar represents 5 um.