Picture from Erdmann, Emily A. et al. (2022) MicroPubl Biol

Figure 1 The effect of the vit-2:gfp transgene on embryo content and brood size:A) Representative DIC images of developmentally synchronized wild-type (WT) and VIT-2::GFP worms. Scale bar = 100 μm. B) Synchronized adult worms from the indicated strains were individually placed in bleach to dissolve the cuticle, and the embryos contained in each worm were counted. Each datapoint represents the average number of embryos per worm from an individual biological replicate of twenty worms. Error bars represent standard error of the mean (SEM). Statistical significance determined by one-way ANOVA. C, D) Synchronized adult worms of the indicated genotypes were moved to fresh NGM plates seeded with Escherichia coli OP50 each day. The offspring on each plate were counted for two consecutive days following hatching. The number of offspring in total (C) and per day (D) were plotted. Datapoints represent technical replicates (with two worms each) for the two biological replicates performed. C) Error bars represent SEM. Statistical significance determined by t-test. **** p value ≤ 0.0001, *** p value ≤ 0.001. D) Day 0 represents L4 stage worms.

Picture from Bayer, Emily A. et al. (2018) MicroPubl Biol

Figure 1 ot900 is a novel null allele of ceh-14. We used gRNAs located in the first and last exons of ceh-14 to generate a large deletion allele spanning the ceh-14 ORF (A). The remaining 35bp in exon 1 are capable of generating an 11aa peptide before a deletion-induced frame-shift results in a premature stop (B).

Picture from Mehle, Emily A. et al. (2020) MicroPubl Biol

Behavioral response of well-fed young adults to aversive chemical stimuli was assessed using the drop assay 20 minutes after transfer to NGM plates lacking bacteria ('off food'). Bar graphs show the percentage of animals responding to the indicated concentrations of (A) the bitter tastant quinine, (B) the heavy metal copper (CuCl2), and (C) the detergent SDS. Inserts represent the corresponding dose-response curves for each genotype across the range of concentrations tested, for readability showing only the means from the bar graphs in panels A-C. n >/= 60 animals for each genotype were assayed at each concentration. Assays were performed on at least three separate days. All tastants were dissolved in M13 buffer, pH 7.4 (Wood 1988). Error bars represent the standard error of the mean (SEM). The one-way ANOVA with Tukey's Honestly Significant Difference (HSD) was used for statistical analysis. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.001, and **** denotes p < 0.0001 compared to wild-type animals. ns denotes p >/= 0.05. F values for 10mM - 1mM quinine, respectively: 16.7, 20.4, 16.1, 5.6, 9.2. F values for 10mM - 0.25mM copper, respectively: 19.9, 22.6, 245.7, 133.8, 212.5, 112.7. F values for 0.1% - 0.0001% SDS, respectively: 29.9, 7.6, 24.0, 11.5

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).