Fig. 3. Quantitative RT-PCR of
rha-1. qRT-PCR was performed on either mRNA or total RNA, as indicated. RNA samples were quantitated using UV absorbance. The number of copies of
rha-1 in the samples was determined using a plasmid containing a cDNA copy of
rha-1 as a standard. The
glp-4(
bn2) worms were grown synchronously to the young adult stage at 25C to prevent proliferation of the gonad. All other worms were grown at 20C. YA: young adult. Bar shows mean values, and the errors bars show one s.e.m.