Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(E, E') Pgrl-9::GFP is expressed in muscle or marginal cells in the terminal bulb of the pharynx.

Picture from Gaglia MM et al. (2012) PLoS Genet "Genes that act downstream of sensory neurons to influence longevity, dauer ...."

A-D. Expression pattern of GFP-fused MCT-1 protein in mct-1p::mct-1::GFP transgenic animals. Bright field image (A) and green fluorescence image (B) of an L4 mct-1p::mct-1::GFP transgenic larva. Magnified bright field image (C) and green fluorescence image (D) of dotted box regions in A and B respectively. E-H. The expression of GFP-fused MCT-1 protein in the mct-1p::mct-1::GFP transgenic animal did not overlap with the signal from amphid sensory neurons labeled with the Di dye. Bright field image (E), green fluorescence image (F), red fluorescence image (G) and overlay of green and red fluorescence images (H) of the head of an L3 mct-1p::mct-1::GFP transgenic animal. Arrowheads indicate cell bodies of amphid sensory neurons that are stained with Di dye. The arrow indicates body wall muscle that expresses a muscle-specific myo-3p::RFP transgene, used as a marker for the generation of the transgenic animals.

Picture from Von Stetina SE et al. (2007) Genome Biol "Cell-specific microarray profiling experiments reveal a comprehensive ...."

(a,e) mec-12::GFP is expressed in touch neurons (arrow) and in specific ventral cord motor neurons (e) at the L2 stage.

Picture from Alexander M et al. (2009) Development "An UNC-40 pathway directs postsynaptic membrane extension in Caenorhabditis ...."

(E-E'') UNC-73B::CFP specifically expressed in muscles in the background of the trIs34 integrated transgene that expresses UNC-40::YFP specifically in muscles. The localization of fusion proteins to the muscle arm termini is indicated with a yellow arrowhead. Scale bars: 50 μm in A-C; 10 μm in E-E''.

Picture from Labed SA et al. (2012) PLoS One "The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene ...."

(A-E) Expression of nipi-4 is seen throughout the epidermis (A & B)), in larvae (C) and adults (A,B,D&E), from head (D) to tail (E), in vulval cells (arrow in B), in rectal cells (arrow in E), but not in the seam cells (arrowhead in A), scale bar 10 um.

Picture from Tilmann C et al. (2005) Dev Cell "Cyclin D regulation of a sexually dimorphic asymmetric cell division."

(D) Wild-type hermaphrodite. (E) Wild-type male.

Picture from Ou CY et al. (2010) Cell "Two cyclin-dependent kinase pathways are essential for polarized trafficking ...."

(E) CDKA-1/p35::YFP expressed in DA9 (wyEx2460).

Picture from Song S et al. (2010) PLoS Genet "A Wnt-Frz/Ror-Dsh pathway regulates neurite outgrowth in Caenorhabditis ...."

(E) cam-1 is expressed in RME neurons (arrow).

Picture from Li P et al. (2016) Neuron "Two Clathrin Adaptor Protein Complexes Instruct Axon-Dendrite ...."

Figure S2. Di-leucine motifs determine polarized protein localization (Asso. w/ Figure 2).(A) Schematic diagram of the C. elegans intestine epithelial cells. The top section shows the basolateral(red) membrane, and the middle section shows the apical (blue) and the basolateral (red) membranes.(B-C) Middle section (B) and top section (C) of GFP-tagged SER-1 localization in intestine epithelial cells.White arrows point to the basolateral membrane. Yellow arrows point to the apical membrane. Scale barsare 10um.(D-E) Middle section (D) and top section (E) of GFP-tagged di-leucine motif mutant SER-1 localization inintestine epithelial cells. White arrows point to the basolateral membrane. Yellow arrows point to the apicalmembrane.(F-G) mCD8-GLR-1C-GFP is co-expressed with cytosolic tdTomato in wild-type (F) and unc-101(m1) (G)animals. Arrows point to the axonal region of the RIA neuron. Scale bar is 10um.(H-I) mCD8-SER-1C(DNSLL)-GFP with the dendritic di-leucine motif is co-expressed with the cytosolic tdTomato in wild-type (H) and unc-101(m1) (I) animals. Arrows point to the axonal region of the RIAneuron. Scale bar is 10um.(J-K) GFP-tagged wild type (J) or mutant GLT-4 (K) is co-expressed with the cytosolic tdTomato in RIA.Arrows point to the dendritic region of the RIA neuron. Scale bar is 10um.

Picture from Yang Y et al. (2017) Dev Cell "Spectraplakin Induces Positive Feedback between Fusogens and the Actin ...."

(E-G) Fluorescence time-lapse images of GFP-tagged EFF-1 (E), F-actin (F), and VAB-10A (G) during the seam-hyp7 cell fusion. Asterisks, V.a.