FIG. 2. Spatial expression pattern of PDI-3 defined by antibody staining and β-galactosidase staining of promoter reporter lines. PDI-3 and DPY-7 antibodies co-staining in the 3-fold- stage embryo (A and B) and the L1 larvae stage (C and D) with magnification X630 are shown. A, PDI-3 antibody staining is observed in numerous tissues including the gut (g) and the hypodermis (h). B, DPY-7 is exclusively localized in the embryonic hypodermal cells. C, PDI-3 antibody staining is observed in the larval gut and the hypodermis; however, the pharynx (p), gut lumen (gl), gut nuclei (gn), and body cavity (bc) are not labeled. D, DPY-7 is exclusively localized in annular furrows (af) of the larval cuticle. E, β-galactosidase staining was observed in lateral seam cell nuclei of transgenic reporter lines containing an extra-chromosomal construct with lacZ gene under the control of the
pdi-3 promoter (X200). The position of the pharynx (p) is denoted. F, transgenic lines also displayed gut cell staining (X100). The position of the pharynx (p) is denoted. The nuclear localization is maintained due to the presence of an nuclear localization signal in the reporter construct. Lateral seam cell nuclear staining (E) and gut cell nuclei staining (F) are indicated by black arrowheads.