Figure 1. The Susm phenotypes of
lon-2,
gpn-1 and
unc-52 mutants: A) Diagram depicting the coelomocyte (CC) phenotype of wildtype (WT),
sma-9(
cc604), or
sma-9(
cc604); susm worms.
sma-9(
cc604) mutants lack the two M-derived CCs located in the posterior of WT or
sma-9(
cc604); susm worms (purple arrows). Created using BioRender.com (version 2021). B) Table showing the penetrance of the Susm phenotype of double mutant strains between
sma-9(
cc604) and mutant alleles of the specified genes (thanks to CGC). The Susm penetrance refers to the percent of animals with one or two M-derived CCs as scored using the CC::GFP reporter. For each genotype, two independent isolates were generated (as shown in the strain list), three to seven plates of worms from each isolate were scored for the Susm phenotype at 20°C, and the Susm data from the two isolates were combined and presented in the table. a The lack of M-derived CCs phenotype is not fully penetrant in
sma-9(
cc604) mutants. Statistical analysis was conducted by comparing the double or triple mutants with the
sma-9(
cc604) single mutants. **** P<0.0001 (unpaired two-tailed Student's t-test). No significant difference was detected between
lon-2(
e678)
sma-9(
cc604) double mutants and
lon-2(
e678)
sma-9(
cc604)
gpn-1(
tm595) triple mutants.