Fig. 5. RNAi of
icp-1 causes de-localization of AIR-2. Wild-type and
icp-1(RNAi) embryos were fixed and stained with anti-tubulin and anti-AIR-2 antibodies and DNA was stained with 4,6-diamidino-2-phenylindole (DAPI). In wild-type embryos, AIR-2 was detected on (a) meiotic chromosomes, and (b) mitotic chromosomes during metaphase and on the spindle midzone during anaphase. In
icp-1(RNAi) embryos at similar stages, discrete localization of AIR-2 is not observed.