Picture from Novelli J et al. (2006) Genetics "The C terminus of collagen SQT-3 has complex and essential functions in nematode ...."

IF staining experiments of N2 worms with the R584 SQT-3-specific serum. (A and B) IF Staining of N2 embryos with the R584 serum. A' and B' are the differential interference contrast (DIC) micrographs of A and B. In comma-stage embryos (A), the reactive antigen is detected intracellularly in a perinuclear distribution. By the late threefold stage (B), all antigen is assembled into the cuticle. Bar, 10 um.

Picture from Lear SK et al. (2018) MicroPubl Biol "Immunofluorescence reveals neuron-specific promoter activity in ...."

In case of the pan-neuronal promoters, expression under cmk-1 promoter is observed in most, if not all neuronal nuclei, including in many head neurons (C top) and ventral nerve cord (VNC) neurons (C bottom).

Picture from Karabinos A et al. (2001) Proc Natl Acad Sci U S A "Essential roles for four cytoplasmic intermediate filament proteins in ...."

The B1 promoter/gfp is expressed in the rectum and some unidentified neurons in mixed-stage animals (H). Whole-mount worms were double-labeled with affinity-purified rabbit antibody specific for IF protein B1 (I) and with antibody MH4 (J).

Picture from Conine CC et al. (2013) Cell "Argonautes promote male fertility and provide a paternal memory of germline gene ...."

(B) Confocal IF images of primary (1) spermatocytes (left) or spermatids (right) in dissected alg-3/4 orWT germlines stained with antibodies against CSR-1 (green) and a nuclear pore protein (red). DNA was stained with DAPI (blue). White arrows denote chromatin domains where CSR-1 localizes.

Picture from Tullet JM et al. (2008) Cell "Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like ...."

(H and I) Substitution of a strongly predicted AKT site within SKN-1A(1-90), Ser12 (Table S1), results in nuclear accumulation of SKN-1A::GFP. In some lines low levels of SKN-1::GFP were observed in additional head neurons, hypodermis, and other tissues, particularly if Ser12 was mutated (data not shown). p values were derived from a chi2 test.

Picture from Karabinos A et al. (2001) Proc Natl Acad Sci U S A "Essential roles for four cytoplasmic intermediate filament proteins in ...."

Figure 4. Expression of A1-, A3-, and B1-promoter/gfp constructs and the IF protein B1. Expression of the A1 promoter/gfp reporter in cells associated with amphid sensory neurons (A), the pharyngeal-intestinal valve (C), the rectum, and some neurons of the tail (E). Similar structures were immunostained with antibody MH4 which, however, also decorates the hypodermis (B, D, F, and J). The A3 promoter/gfp reporter is primarily expressed in the embryonal and larval hypodermis (G). The B1 promoter/gfp is expressed in the rectum and some unidentified neurons in mixed-stage animals (H). Whole-mount worms were double-labeled with affinity-purified rabbit antibody specific for IF protein B1 (I) and with antibody MH4 (J). [Scale bars: 50 um (A, C, E, and H) and 25 um (B, D, F, G, I, and J).]

Picture from Lee KK et al. (2000) Mol Biol Cell "C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins ...."

Immunofluorescence localization of Ce-lamin at different stages of mitosis in 2- to 24-cell C. elegans embryos (A) and 30-cell C. elegans embryos (B). Embryos were doubly stained for DNA with the use of Hoechst 33258 (D) and by indirect immunofluorescence (IF) with the use of antibodies specific for Ce-lamin. A representative nucleus from each stage is shown: INT, interphase; PRO, prophase; PROM, prometaphase; META, metaphase; ANA, anaphase; and TELO, telophase.

Picture from Novelli J et al. (2006) Genetics "The C terminus of collagen SQT-3 has complex and essential functions in nematode ...."

Figure 5. IF staining experiments of N2 worms and sqt-3(e2924, e2117) mutants with the R584 SQT-3-specific serum. (A and B) IF Staining of N2 embryos with the R584 serum. A' and B' are the differential interference contrast (DIC) micrographs of A and B. In comma-stage embryos (A), the reactive antigen is detected intracellularly in a perinuclear distribution. By the late threefold stage (B), all antigen is assembled into the cuticle. (C) e2924 embryos labeled with the R584 serum. No specific signal is detected. The puncta represent background fluorescence. (D and E) Staining of e2117 embryos with the R584 serum. D' and E' are the DIC micrographs of D and E. The animals in D and E were raised at 15 and at 25, respectively. In both cases, SQT-3 failed to be secreted. The animal in D is a threefold-stage embryo, while the one in E is an arrested L1 larva. Note the persistence of the retained collagens in the hypodermis of the L1 specimen (E, arrowheads). Bars, 10 um.

Picture from Lee KK et al. (2000) Mol Biol Cell "C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins ...."

Immunofluorescence localization of Ce-emerin at different stages of mitosis in 2- to 24-cell C. elegans embryos (A) and 30-cell C. elegans embryos (B). Embryos were doubly stained for DNA with the use of Hoechst 33258 (D) and by indirect immunofluorescence (IF) with the use of antibodies specific for Ce-emerin. A representative nucleus from each stage is shown: INT, interphase; PRO, prophase; PROM, prometaphase; META, metaphase; ANA, anaphase; and TELO, telophase. All Ce-emerin immunofluorescence images were obtained by confocal microscopy.

Picture from Lear SK et al. (2018) MicroPubl Biol "Immunofluorescence reveals neuron-specific promoter activity in ...."

In case of the pan-neuronal promoters, expression under unc-119 promoter is observed in most, if not all neuronal nuclei, including in many head neurons (D top) and ventral nerve cord (VNC) neurons (D bottom). However, in a subpopulation of animals expressing BFP under the unc-119 promoter, intense fluorescence was observed in several additional larger nuclei along the lateral sides (D, blue arrowheads), which we identify as hypodermal cells and faint signals in even larger nuclei (D, pink arrowheads), which we identify as intestinal cells.