Picture from Chien E et al. (2023) MicroPubl Biol "Transcriptional repression during spermatogenesis in C. elegans ...."

Figure 1. Diplotene/diakinesis spermatocytes treated with top-2, capg-2, and met-2 RNAi exhibit increased RNAPIIpSer2 signals.:A. Dissected wild-type male gonads were fixed and stained for DNA (blue). The gonad is divided into three regions based on DNA morphology. The compaction zone, comprised of diplotene and diakinesis spermatocytes, was examined in subsequent experiments. Scale bar equals 10 μM.B. Gonads from male animals treated with control RNAi were fixed and stained for RNAPII (pSer2; red) and DNA (blue). Representative image of the compaction zone (C.Z.) is shown. Scale bar equals 10 μM.C. Gonads from male animals treated with capg-2 RNAi were fixed and stained for RNAPII (pSer2; red) and DNA (blue). Representative image of the compaction zone (C.Z.) is shown. Scale bar equals 10 μM.D. Gonads from male animals treated with top-2 RNAi were fixed and stained for RNAPII (pSer2; red) and DNA (blue). Representative image of the compaction zone (C.Z.) is shown. Scale bar equals 10 μM.E. Gonads from male animals treated with met-2 RNAi were fixed and stained for RNAPII (pSer2; red) and DNA (blue). Representative image of the compaction zone (C.Z.) is shown. Scale bar equals 10 μM.F. Quantification of data presented in panels B-E. RNAPIIpSer2 signal was measured using ImageJ. The average ratio of normalized raw integrated density is plotted on the y-axis, and this corresponds to the ratio of signal intensities derived from the compaction zone (C.Z.) over the pachytene zone. Samples were analyzed for each RNAi treatment over at least 2 independent replicates. Significance was measured using Wilcoxon Rank Sum test. The number of samples analyzed for each condition is shown below. **p<0.01; *p<0.05.

Picture from Carman, Lynly et al. (2022) MicroPubl Biol

Figure 1. An AMPK biosensor for Caenorhabditis elegans:A. Phospho-AMPKα (Thr172) antibodies bind to wild-type AAK-2 but not AAK-2 from aak-2(T243A) and aak-2(S244G) animals.B. Schematic of the AMPKAR-EV biosensor structure and function.C. Representative image of nhx-2p::ceAMPKAR-EV, rab-3p::ceAMPKAR-EV, and myo-3p::ceAMPKAR-EV transgenic animals. The defined ROI (box) shows where the FRET analyses were conducted, scale bar is 25µm.D. Paraquat and metformin exposure resulted in a significant increase in the normalized FRET ratio in the intestine over a period of 30 minutes (*p<0.05 at all points within the bracket; N=10). Statistical significance was calculated using two-way ANOVA followed by Sidak's multiple comparisons test.E. Wild-type animals pre-treated with paraquat and metformin exhibit a significant increase in normalized FRET in their intestine over time (*p<0.05 including all points within the bracket; N=10). Statistical significance was calculated using two-way ANOVA followed by Sidak's multiple comparisons test.F. aak-2(T243A) and aak-2(S244G) animals showed no difference in normalized FRET in their intestine over time between treated and control animals (N=10). Statistical significance was calculated using two-way ANOVA followed by Sidak's multiple comparisons test.G. Wild-type animals that were pre-treated with paraquat and metformin exhibited a significant increase in normalized FRET in a tail neuron (DVA) over time (*p<0.05 at all points within the bracket; N=10). Statistical significance was calculated using two-way ANOVA followed by Sidak's multiple comparisons test.H. Wild-type animals pre-treated with paraquat and metformin did not exhibit any changes in normalized FRET in a body wall muscle compared to controls (N=10). Statistical significance was calculated using two-way ANOVA followed by Sidak's multiple comparisons test.