Figure 1. Structure of the
ptp-3 locus. (A) Genomic structure of the
ptp-3 locus and location of
op147::Tc1. The long transcript, encoding PTP-3A, is generated from 29 coding exons that span 36.2 kb of genomic DNA; this transcription unit is denoted C09D8.1 in the C. elegans genome database (also previously known as clar,
ypp-1, and
ptp-1). Four large introns are not shown to scale (dotted lines). Exons 1-13 are specific to the long transcript. Exon 14 contains the ATG for the short transcript and is transcribed from an internal promoter. Exon 13 (PTP-3A) or exon 14 (PTP-3B) both splice to exon 15. Exons 15-30 are common to both transcripts. The transmembrane domain (tm) and the D1 phosphatase domain are encoded by exon 26. The Tc1 insertion of
op147 is in exon 26, disrupting the D1 phosphatase domain. The PstI site used to insert GFP is indicated. The genomic region contained in cosmid clone F38A3 is indicated. (B) Northern blot of total C. elegans mRNA extracted from mixed-stage populations, probed with the PTP-3B cDNA (see Materials and Methods). Messages of approx. 8 kb and approx. 5 kb are detected. (C) Partial genetic map of the center of linkage group II, showing map location of
ptp-3,
vab-1, and markers used in strain constructions.