Picture from Wissmann AK et al. (1997) Genes Dev "Caenorhabditis elegans LET-502 is related to Rho-binding kinases and human ...."

Figure 5. Expression of let-502 reporter constructs. b-Galactosidase staining in transgenic let-502::lacZ animals. Embryos were analyzed by indirect immunofluorescence after simultaneous staining with three monoclonal antibodies. MH27 stains the belt desmosomes outlining the hypodermal cells (short white arrows; Francis and Waterston 1991). DM5.6 stains body wall muscle myosin located in muscle quadrants just beneath the hypodermal cells (long white arrows; Miller et al. 1983). The dorsal left (A) and the dorsal left and right (B) muscle quadrants are visible in the focal planes shown. Anti-b-galactosidase antibody stains either the nuclei of the seam cells (nuclear localized let-502::lacZ reporter construct) as shown in A or the seam cell cytoplasm (non-nuclear localized let-502::lacZ reporter construct) as shown in B. One staining seam cell for each embryo is indicated by a black arrow. Occasional seam cells do not stain for b-galactosidase, presumably because of mosaic loss of the extrachromosomal element containing the reporter constructs. Less intense staining in other hypodermal cells can also be detected (data not shown). Embryos are oriented with dorsal at the top and anterior to the left. Both wild-type embryos are at early elongation, with B presenting a more dorsal aspect than A (see Fig. 1). Bar, 10 um.

Picture from Podbilewicz B (1996) Mol Biol Cell "ADM-1, a protein with metalloprotease- and disintegrin-like domains, is ...."

Figure 8. Expression of ADM-1 inmature sperm and in the lumen of the pharynx and buccal cavity. Immunofluorescence images of sperm and embryos using the rat monoclonal antibody 17B7 raised against the cytoplasmic tail of ADM-1. (A) Staining of sperm released from males. Arrows point to two sperm cells; all of the immunoreactivity in this micrograph correspond to sperm bound to the slide. C. elegans spermatozoa are non flagellated crawling cells with a single pseudopod.(B) Same field as in A shows seven nuclei stained with propidium iodide and containing condensed chromatin.The nucleus of the sperm in the center is in a different focal plane. (C) Expression of ADM-1 in the luminal domain of the pharynx and buccal cavity in threefold elongating embryos. (D) The pharynx in the same threefold embryo of C stained with the mouse mono- clonal antibody 3NB12.13. (E) Expression of ADM-1 in the apical domain of the pharynx and buccal cavity.(F) The same embryo as in E stained with mouse monoclonal antibody MH27 (Francis and Waterston, 1991).The MH27 antibody was used to precisely identify the position and identity of the cells that are stained with 17B7; arrows indicate the first and second bulbs of the pharynx (right and left,respectively).b, buccal cavity; i,intestine. Micrographs were obtained with a MRC-1024 confocal microscope. C-F are projections of 15-20 optical sections (Podbilewicz and White, 1994). Bars, 10 ,um (A and B); 10 ,um (C-F).

Picture from Nilsson L et al. (1998) Development "Caenorhabditis elegans lin-25: cellular focus, protein expression and ...."

Cellular expression and sub-cellular distribution of the LIN-25 protein. Hermaphrodites in the left column (C,E,G) were stained with MH27 antibody (which stains adherens junctions (Francis and Waterston, 1991) and hermaphrodites in the right column were stained with anti-LIN-25 antiserum 4478 (D,F,H). (C-H) Worms that overexpress LIN-25 from the integrated array, svIs1. (C,D) Arrowheads indicate the positions of the VPCs, arrows indicate the positions of the seam cells and other lateral hypodermal cells. The line in D indicates the position of the central part of the somatic gonad. Note that in C there are far more lateral hypodermal cells than seen in wild-type worms (not shown). (E,F) Absence of staining in the descendents of P4.p and P8.p. Anterior is to the left. Open arrowheads point to P4.pa and P4.pp (anterior arrowhead) and P8.pa and P8.pp (posterior arrowhead). The MH27 staining of the P4.p and P8.p descendents is still weakly visible in E but no LIN-25 staining of these cells is visible in F. Expression of LIN-25 in P5.ap is clearly visible in F. Expression of LIN-25 by the other descendents of P5.p, P6.p and P7.p is largely obscurred by the staining of the underlining somatic gonad (indicated by a line). Arrows point to seam cells. (G,H) Staining of P5.p, P6.p and P7.p descendents after two rounds of division. Line in H indicates staining of the somatic gonad.

Picture from Kachur T et al. (2004) J Cell Sci "Maternal UNC-45 is involved in cytokinesis and colocalizes with non-muscle ...."

Figure 1. UNC-45 expression in the nematode Caenorhabditis elegans adult germline and early embryo. (A) A hermaphrodite gonad doubly stained with anti-UNC-45 antibody (green) and DAPI (blue), which stains the cell nuclei. (B) Distal part of hermaphrodite gonad doubly stained with pre-immune serum and DAPI as controls. (C,D) Two-cell (C) and 16-cell (D) embryos stained with anti-UNC-45. Arrows indicate concentration of UNC-45 at cell boundaries. In panels E-H, embryos are doubly stained for UNC-45 (E,G) and monoclonal MH27 (F,H), which recognizes an epitope associated with adherent junctions at the boundaries of hypodermal cells (Francis and Waterston, 1985; Hresko et al., 1994). As the embryo begins to elongate, the UNC-45 staining is progressively restricted to the presumptive muscle cells (G, arrowhead). (I,J) GFP and DIC images of a two-fold embryo transgenic for an UNC-45::GFP fusion protein, showing expression in the body wall muscles (arrowhead). (K-N) UNC-45 staining is detectable throughout embryogenesis whereas MHC-B staining is only detectable in the developing body wall muscles. K and M are stained with anti-UNC-45, L and N with anti-MHC B (5-8). (K,L) The same embryo at about 250 minutes after fertilization. (M,N) The same embryo at the 1.5-fold stage of elongation. The images of L and N were exposed for the same times, serving as controls to show that although MHC B does not show staining in the early embryos, UNC-45 does.

Picture from Nilsson L et al. (1998) Development "Caenorhabditis elegans lin-25: cellular focus, protein expression and ...."

Figure 5. Cellular expression and sub-cellular distribution of the LIN-25 protein. Hermaphrodites in the left column (A,C,E,G) were stained with MH27 antibody (which stains adherens junctions (Francis and Waterston, 1991) and hermaphrodites in the right column were stained with anti-LIN-25 antiserum 4478 (B,D,F,H). (A,B) lin-25(ar90) mutant worms. (A) Arrowheads indicate the positions of the VPCs. (C-H) Worms that overexpress LIN-25 from the integrated array, svIs1. (C,D) Arrowheads indicate the positions of the VPCs, arrows indicate the positions of the seam cells and other lateral hypodermal cells. The line in D indicates the position of the central part of the somatic gonad. Note that in C there are far more lateral hypodermal cells than seen in wild-type worms (not shown). (E,F) Absence of staining in the descendents of P4.p and P8.p. Anterior is to the left. Open arrowheads point to P4.pa and P4.pp (anterior arrowhead) and P8.pa and P8.pp (posterior arrowhead). The MH27 staining of the P4.p and P8.p descendents is still weakly visible in E but no LIN-25 staining of these cells is visible in F. Expression of LIN-25 in P5.ap is clearly visible in F. Expression of LIN-25 by the other descendents of P5.p, P6.p and P7.p is largely obscurred by the staining of the underlining somatic gonad (indicated by a line). Arrows point to seam cells. (G,H) Staining of P5.p, P6.p and P7.p descendents after two rounds of division. Line in H indicates staining of the somatic gonad.

Picture from Hoppe PE et al. (2010) Genetics "Caenorhabditis elegans unc-82 encodes a serine/threonine kinase important for ...."

Figure 5. A rescuing UNC-82::GFP fusion protein is localized near the M-line in body-wall muscle. (A-C) Portions of two body-wall muscle cells from an adult unc-82(e1323) homozygote expressing UNC-82::GFP are shown stained with antibodies against (A) UNC-89/obscurin and (B) GFP. The signals coincide in the merged image (C). The asterisks (A) mark the boundary between the two muscle cells, and the arrows indicate one end of each muscle cell. (D-F) One plane from a z-series in which UNC-82::GFP was detected throughout the depth of the myofilament lattice, as marked by a-actinin (Francis and Waterston 1985; see Figure 1). (G-I) A 1.5-fold embryo, positioned as in Figure 2A, exhibits punctate UNC-82::GFP concentrated near myosin in a single muscle quadrant (arrows). Asterisks mark the posterior tip of the embryo. (J and K) The e1323 embryo on the left (arrow marked '+') expresses UNC-82::GFP, whereas the embryo on the right (arrow marked '-') has lost the unstable transgene. (J) The myosin staining (between asterisks) appears broken and patchy in the mutant without the transgene (-). Anti-GFP signal (K) outside the body-wall muscle quadrants (box in J and K) in only the rescued embryo (+) suggests fusion protein expression in nonmuscle cells. An adult pharynx (L-N) exhibits no overlap between the UNC-89 and UNC-82::GFP signals. (L) UNC-89 signal marks the regions of the single-sarcomere pharyngeal muscle cells that contain thick filaments. The UNC-82::GFP signal (M, arrow in N) appears localized to the marginal cells or to a region of the muscle cell that does not contain thick filaments. (N) The pharynx shown is bent back in the isthmus (i) such that the procorpus (pc) lies alongside the terminal bulb (tb). The antibodies used were anti-UNC-89, MH42; rabbit anti-GFP, Abcam; antimyosin A, 5.6; anti-a-actinin, MH35. Bar, 5 um.

Picture from Greenstein D et al. (1994) Genes Dev "Targeted mutations in the Caenorhabditis elegans POU homeo box gene ceh-18 cause ...."

Fig 5. Immunofluorescence detection of CEH-18-expressing cells. CEH-18-expressing cells were detected using anti-CEH-18Amg57 antibodies to stain wild type (a,c,g,i), or ceh-18(mg57) (e). Nuclei were also visualized with DAPI (b,d,f,h,j). The same samples are shown in a and b (dissected gonad of adult hermaphrodite), c and d(wild-type threefold-stage embryo(, e and f (ceh-18 (mg57) three fold stage embryo), g and h (wild-type 12-cell embryo), i and j (wild-type -150-cell embryo). There are five pairs of gonadal sheath cells per gonad arm, designated 1 - 5 (see Fig. 3). Only one of each pair is shown; the other five nuclei are out of the plane of focus but also stain. The DAPI image in b i s in a slightly lower focal plane to show the diakinesis stage nuclei in the proximal arm of the gonad (indicated by arrows). The arrows mark CEH- 18 -expressing gonadal sheath cell nuclei, which are also numbered. The distal arm of the gonad containing pachytene stage and mitotic germ cell nuclei is indicated by an arrow. Sperm nuclei are indicated. The background in the germ-line cytoplasm is attributable to the secondary antibody. No staining was seen in ceh-18 (mg57) at any stage of development (see text). Note CEH-18 is first expressed late in embryogenesis at the threefold stage, before hatching, and is absent from early embryos. At hatching, CEH-18 is most abundant in the pharyngeal muscle nuclei, the nuclei of the hypodermal syncytia (hyp 1-11), the nuclei of the seam cells, the P hypodermal blast cells, and the body muscle nuclei. CEH-18 is notably absent from nearly all neurons at this stage, although it is expressed by the anterior touch cells ALML/R. Bars, 50 and 25 um, in a and c, respectively. a and b were kindly provided by R. Francis and T. Schedl (Washington University School of Medicine, St. Louis, MO).