Picture from Clark-Maquire SD et al. (1994) Genetics "mei-1, a gene required for meiotic spindle formation in Caenorhabditis elegans, ...."

FIGURE 5. Northern blot analysis of mei-1 mRNA. Approximately equal amounts of polyA+ mRNA were loaded in each lane. A fragment of pHR8, from the EcoRV site at 942 in Figure 2 to the end of the coding sequence, was used as the probe.

Picture from Berkowitz LA et al. (2000) Development "MES-1, a protein required for unequal divisions of the germline in early C. ...."

Figure 3. Northern analysis of mes-1 transcripts. (A) mes-1 mRNA is detectable in wild type (N2) and undetectable in the mes-1 deletion allele bn74. (B) mes-1 mRNA accumulation during development. PolyA+ RNA was isolated from wild-type hermaphrodites, whichwere synchronized at each of the six developmental stages shown. A, adults; E, embryos; L1-L4, four larval stages. In (A) and (B) a 2.6 kb partial cDNA clone of mes-1 was used as a probe to detect the 3.7 kb transcript. The transcript of the ribosomal gene, rpp-1, was used as a loading control. Relative levels of the mes-1 transcript are shown at the bottom.

Picture from Marson AL et al. (2001) Gene "Macrophage migration inhibitory factor (mif) transcription is significantly ...."

Fig. 3. (A) Stage-associated transcription of Ce-mifs. PolyA+ mRNA was isolated from eggs, L1, L2, L3, L4 and adult worms, converted to single-stranded cDNA and used as template in PCR. Gene-specific primers were used to amplify the open reading frames of mif-1, mif-2, mif-3 or C.elegans nucleoside diphosphate kinase (ndk). PCR conditions were 94C for 30 s, 56C for 45 s, and 72C for 45 s for a total of 25 cycles for Ce-ndk and 30 cycles for all Ce-mif genes. PCR products were electrophoresed on a 1.2% agarose gel and stained with ethidium bromide. (B) Dauer-specific gene expression. cDNA from equivalent numbers of L2 worms, dauers, and 18-hour post-dauer worms were used as template in real-time quantitative PCR using the SYBR Green Assay. Gene-specific primers were used for each gene: ndk (nucleoside diphosphate kinase), Ce-mif-1, Ce-mif-2, Ce-mif-3, and col-1 (collagen-1). The data were expressed as the relative quantity of transcripts using the gene-specific transcription levels at the L2 stage as baseline. All of the samples were normalized to the expression levels of the constitutatively expressed gene ndk.