Figure 2. (left). Expression pattern and cell nonautonomous activity of
unc-129. (A) Comparison ofunc-129::gfp transcriptional reporters used in this study with
unc-129 rescuing genomic DNA. (Bto E)
unc-129::gfp expression pattern at ~280 min (B), ~430 min (C), ~550 min (D) postcleavage, and in LI larvae (E). Expression in dorsal body wall muscle [arrowheads in (D) and (E)] is observed before DA and DB expression. GFP expression vectors used in (B) to (D) contain a nuclear localization signal. (F and G)
unc129::gfpreporter derivatives expressed predominantly in DA and DB motorneurons (F) (arrow indicates dorsal nerve cord) and dorsal body wall muscle in L1 larvae (G). (H)
unc-129 expression from the dorsal muscle-specific promoter is sufficient to completely rescue theunc-129 phenotype. Restriction sites as in Fig. 1. Bar, 10 um.