Picture from Sepulveda, Nicholas B et al. (2021) MicroPubl Biol

Figure 1. JU1171, LKC34, and N2 males were less likely to mate successfully and produced significantly fewer viable cross progeny at 27°C: Males were either raised at 20°C (blue bars) or upshifted to 27°C at L1 stage (red bars) and crossed with either strain matched or unc-24(e138) (Unc) hermaphrodites. (A-C) The percentage of males that mated successfully and produced viable cross progeny (% Successful males) was assessed. Significantly fewer males of all three wild type strains mated successfully to produce viable cross progeny at 27°C versus 20°C (Fisher's Exact Test, *P < 0.05). (D-F) The number of viable cross progeny produced by successful males was assessed (dead eggs and unfertilized oocytes were not counted). Males of all three wild type strains produced significantly fewer viable cross progeny at 27°C versus 20°C (Two-way ANOVA with Tukey's correction, *P < 0.05). Error bars represent ± 1 SEM.

Picture from Wirshing ACE et al. (2018) Mol Biol Cell "Spectrin regulates cell contractility through production and maintenance of ...."

(C) Confocal maximum intensity projection and a sagittal cross sectionof a spermatheca expressing labeled SPC-1/alpha and AJM-1 to label apical junctions. (C'') Insertfrom the cross section in (C) indicated by a white box with apical at the top and basal at thebottom. SPC-1/alpha is most prominent at the lateral cell membrane and extends down to the apicaljunction. A thin layer of SPC-1/alpha is visible at the basal cell surface (white arrowheads). (D)Confocal maximum intensity projection and a sagittal cross section of a spermathecaexpressing labeled SPC-1/alpha and GFP::UtrCH to label F-actin. (D'') Insert from the cross sectionin (D) with apical at the top and basal at the bottom. The thin layer of SPC-1/alpha at the basal cellsurface overlaps with basal actin bundles (white arrowheads). Scale bar 20 um, 10 um ininserts

Picture from Shao Z et al. (2013) Cell "Synapse location during growth depends on glia location."

(A) A larval animal displaying the endogenous cima-1 expression pattern as determined by rescuing construct CIMA-1(genomic)::SL2::GFP. The dashed box corresponds to the region where AIY is located (AIY not shown; this region is also similar to the region examined in [B] and [C]). Arrows point at CIMA-1-expressing epidermal cells. (B-D) Simultaneous visualization of cima-1(genomic)::SL2::GFP (green) and body wall muscle reporter Pmyo-3::mcherry (red). Note the nonoverlapping expressing pattern of cima-1 in epidermal cells and Pmyo-3::mcherry in muscles, both in the sagittal cross-section (B) and in the transverse cross-section (C). Dashed line in (B) indicates site of transverse cross-section image in (C). And (D) is the schematic drawing of (C), with muscle quadrants (M), epidermal cells (green) and pharynx (P) labeled. Scale bars, 10 um.

Picture from Gleason AM et al. (2016) Mol Biol Cell "Syndapin/SDPN-1 is required for endocytic recycling and endosomal actin ...."

Supplemental Figure S1. Expression profile of SDPN-1 in C. elegans. Confocal images of the (A) pharynx, (B) intestine (top, en face basal view), and (C) intestine (middle, cross sectional view) arrows indicate the apical intestinal membrane. Scale bar 10 um.

Picture from Sheth U et al. (2010) Development "Perinuclear P granules are the principal sites of mRNA export in adult C. elegans ...."

Figure 5. NXF-1 is concentrated at Pg-NPCs. (A) Gonad and intestine (inset) co-stained for NPP-9 and NXF-1 as indicated. (B) Surface view of a single pachytene germ nucleus (outlined) stained for NXF-1 and PGL-1 as indicated; note extensive association of the two proteins (arrow). (C) Optical cross-section of a single pachytene nucleus stained for NXF-1, PGL-1 and DNA as indicated. Note NXF-1 localization to the base of P granules (arrow) and in the nucleolus (arrowhead). (D) Optical cross-section of a single pachytene nucleus stained for NXF-1, NPP-9 and DNA as indicated. Scale bars: 25 μm in A; 2.5 μm in B-D.

Picture from Macrae M et al. (1995) Genetics "The ornithine decarboxylase gene of Caenorhabditis elegans: cloning, mapping ...."

Figure 5. stage dependent odc activity. Eggs were cultured and the resulting synchronized population ssampled periodically, scored by microscopic examination to assess the predominate developmental stage and extracts prepared for determination of ODC enzymatic activity. Activity was determined after preincubation in the absence (cross-hatched bars) or presence (open bars) of DFMO, a specific inhibitor of enzymatic activity.

Picture from Benian GM et al. (1996) J Cell Biol "The Caenorhabditis elegans gene unc-89, required fpr muscle M-line assembly, ...."

Figure 5. EU30 and MH42 localize to the middle of A-bands in both body wall and pharyngeal muscle from wild-type animals. (A and B) Animals were simultaneously reacted with a polyclonal ct-twitchin and monoclonal MH42, and secondary antibodies of goat ct-rab- bit-fluorescein and goat a-mouse rhodamine as described in Materials and Methods. A portion of body wall muscle is shown, as viewed with the fluorescein channel in A (to show et-twitchin reaction), and the rhodamine channel in B (to show MH42 reaction). The arrows point to the middle of the same muscle A-band. (C and D) Animals were simultaneously reacted with a monoclonal to myosin heavy chain A (ct-myoA) and polyclonal EU30, and appropriate fluor-labeled secondary antibodies. The same portion of body wall muscle is shown, as seen with the rhodamine channel in C (to reveal a-myoA reaction) and as seen with the fluorescein channel in D (to reveal EU30 reaction). (E and F) Frozen cross sections were simultaneously reacted with a monoclonal to pharyngeal muscle-specific myosin heavy chain C (et-myoC; shown in E), polyclonal EU30 (shown in F), and appropriate fluor-labeled secondary antibodies. E and F are from the same cross section of a pharynx as viewed with the rhodamine and fluorescein channels, respectively. In the cross-sections in F and G (below), EU30 and MH42 also react with the four body wall muscle quadrants. (G) Frozen cross section reacted with monoclonal MH42 and goat a-mouse-rhodamine. Bars, 5 um.

Picture from Wirshing ACE et al. (2018) Mol Biol Cell "Spectrin regulates cell contractility through production and maintenance of ...."

Figure 4: SPC-1/alpha-spectrin, UNC-70/beta-spectrin and SMA-1/beta heavy-spectrinhave distinct expression patterns in the spermatheca and SP-UT valve. (A) Confocalmaximum intensity projections of whole animals expressing labeled SPC-1/alpha, UNC-70/beta, andSMA-1/betaH. Yellow arrow indicates location of the spermatheca. (B) Confocal maximum intensityprojections of excised and fixed spermatheca from L4 and young adult animals expressinglabeled SPC-1/alpha, UNC-70/beta, and SMA-1/betaH. The spermatheca and SP-UT valve are outlinedwith a dashed line. Note SMA-1/betaH is restricted to a band connecting the spermathecal bag andSP-UT valve in the adult spermatheca (white arrow). Images are colored to highlight differencesin fluorescence intensity. (C) Confocal maximum intensity projection and a sagittal cross sectionof a spermatheca expressing labeled SPC-1/alpha and AJM-1 to label apical junctions. (C'') Insertfrom the cross section in (C) indicated by a white box with apical at the top and basal at thebottom. SPC-1/alpha is most prominent at the lateral cell membrane and extends down to the apicaljunction. A thin layer of SPC-1/alpha is visible at the basal cell surface (white arrowheads). (D)Confocal maximum intensity projection and a sagittal cross section of a spermathecaexpressing labeled SPC-1/alpha and GFP::UtrCH to label F-actin. (D'') Insert from the cross sectionin (D) with apical at the top and basal at the bottom. The thin layer of SPC-1/alpha at the basal cellsurface overlaps with basal actin bundles (white arrowheads). Scale bar 20 um, 10 um ininserts

Picture from Bercher M et al. (2001) J Cell Biol "mua-3, a gene required for mechanical tissue integrity in Caenorhabditis ...."

Figure 4. Localization of MUA-3 to hemidesmosome zone of hypodermis in animals stained with anti-MUA-3 antibodies. (A) Immunofluorescence in hypodermis-bordering muscle quadrants using MUA-3 antibody recognized with a FITC secondary antibody. a, ALM; b, hemidesmosome region of hypodermis; c, gaps where nerve processes cross between hypodermis and muscle. (B) Enlargement of hemidesmosome region of hypodermis. Bars, 10 um.

Picture from Cueva JG et al. (2007) J Neurosci "Nanoscale organization of the MEC-4 DEG/ENaC sensory mechanotransduction ...."

Figure 3. Nanoscale position of the MEC-2 and MEC-4 MeT channel subunits and MEC-5 collagen in wild-type TRNs. A , Schematic cross-section showing the relative positions of the cuticle (gray), four muscle quadrants (green), hypodermis (brown), PLM (green), PLN (blue), and ECM (purple). Structures not to scale. Adapted from WormAtlas (Altun and Hall, 2005). B , Electron micrograph showing a cross-section through the cuticle (gray), ECM (purple), PLM (blue), and PLN (green). Cuticle (apical) side is up. Scale bar, 200 nm. C , Immunoelectron micrographs of thin sections labeled with anti-MEC-2 (top), anti-MEC-4 (middle), and anti-MEC-5 (bottom). Insets are digitally magnified (2×) views of each label cluster. Filled arrowheads indicate gold beads; open arrowhead indicates a mitochondrion in PLM. Scale bar, 200 nm. D , Circumferential position of MEC-2 (top), MEC-4 (middle), and MEC-5 (bottom). Symbol size is proportional to the number of gold beads in a cluster in each section; the smallest symbol corresponds to a single gold bead and the largest symbol corresponds to four gold beads. The average ECM density for the labeled sections is shown as a grayscale annulus around the neurite cross-section. E , MEC-5 collagen but not MeT channel subunits exhibit an apical bias. *p < 0.01, χ2 test. The number of labels in each category is indicated in parentheses.