Picture from Henderson ST et al. (1997) Mol Biol Cell "Functional domains of LAG-2, a putative signaling ligand for LIN-12 and GLP-1 ...."

Figure 3. LAG-2(extra 1-286) induces germ line tumors and ectopic GLP-1. A composite of confocal images of dissected adult hermaphrodite germ lines is shown; distal is left, stained with antibodies to GLP-1 (Crittenden et al., 1994). (A) Wild-type germ line. GLP-1 is associatedwith membranes at distal end of the germ line and is absent proximally. Inset, arrow indicates faint staining at the distal tip of the germ line.(B) +;Ex LAG-2(extra 1-286) germ line, raised at 25C. GLP-1 is present both distally and proximally. Inset, open arrow indicates intenseGLP-1 staining associated with the distal tip of the germ line; closed arrows indicate intense punctate staining within the germ line. Scale bar,20 um.

Picture from Henderson ST et al. (1994) Development "lag-2 may encode a signaling ligand for the GLP-1 and LIN-12 receptors of C. ...."

Figure 6. Distribution of LAG-2::β-gal and GLP-1 in adult germline.(A) Schematic representation of distal region of adult germline, showing DTC on left. DTC nucleus shown as open circle, mitotic germ nuclei shown as black circles. Thin lines represent cell membranes, mitotic germ line is a syncytium. Scale bar, 20 µm. (B-D) Confocal images of dissected germlines that have been double stained with both anti-β-galactosidase (red) and anti-GLP-1 (green)antibodies. (B) GLP-1 is present in germline membranes. See Crittenden et al. (accompanying paper) to see definitively that the DTC does not contain GLP-1. (C) The LAG-2::β-gal fusion protein is present in the DTC and also near the center of the germline. (D) Simultaneous detection of anti-GLP-1 and anti-β-gal using merged fluorescein and rhodamine confocal image. Note co-localization of GLP-1 staining and β-gal staining within membranes of the germline.