Figure 9. Endogenous CEH-22 protein and a
ceh-22::lacZ gene fusion are expressed in developing pharyngeal muscle. (A-C) Immunofluorescent localization of endogenous CEH-22 protein using affinity-purified
c187 anti-CEH-22 antibodies. Examples of a lima bean stage embryo (A), a 1G-fold embryo (B) and a pretzel stage embryo (C) are shown. The developmental stage of stained embryos is inferred from the degree of morphogenesis: lima bean embryos have just completed the bulk of cell divisions; 1G-fold and pretzel stage are progressive stages of embryonic elongation. (D-F) Expression of
aceh-22::lacZ fusion detected by in situ staining with X-gal. Examples of -gal expression in a bean stage embryo (D), 1G-fold embryo (E) andadult (F) are shown. In the pretzel stage embryo (C) and the adult (F), the positions of CEH-22-positive or -gal-positive
m3,
m4,
m5 and
m7nuclei are indicated. The
ceh-22::lacZ fusion is integrated into a chromosome; however -gal expression in many animals is mosaic. In a largenumber of stained animals expressing this
ceh-22::lacZ fusion, no activity outside of pharyngeal muscle has been observed.