Picture from Kim JS et al. (2010) Development "C. elegans STRADalpha and SAD cooperatively regulate neuronal polarity and ...."

(A) The expression of a Pstrd-1-nls::gfp reporter in the pharynx, excretory canal and the entire nervous system. (D) Sub-cellular localization of GFP-tagged, functional STRD-1 in GABAergic neurons. GFP::STRD-1 localized to the cytoplasm and the nucleus as well as along both nerve cords. Scale bars: 5 μm.

Picture from Steger KA et al. (2004) Genetics "The GAR-3 muscarinic receptor cooperates with calcium signals to regulate ...."

Figure 2. gar-3 encodes a muscarinic acetylcholine receptor expressed in neurons and in the pharyngeal muscle. (A) Alignment of the N-terminal portion of the GAR-3 amino acid sequence with human muscarinic receptors (the GAR-3 sequence data are available in the GenBank databases under accession no. AF139093). The vu78 point mutation is indicated by an arrowhead. The lg1201 deletion is underlined. The deletion also disrupts the intron-exon junctions identified by Hwang et al. (1999) so that sequences 3' to the breakpoints are likely mistranslated. A complete alignment is available in the supplementary information at http://www.genetics.org/supplemental/. (B) A gar-3 reporter is expressed in neurons of the extrapharyngeal nervous system; in the pharyngeal muscles of the metacorpus, isthmus, and terminal bulb; and in pharyngeal neuron I3 (obscured by muscle staining). Bar, 50 um.

Picture from Yee LE et al. (2015) PLoS Genet "Conserved Genetic Interactions between Ciliopathy Complexes Cooperatively ...."

(B) GFP-tagged TCTN-1 localizes specifically to the transition zones (TZs) in head (amphid) and tail (phasmid) cilia of C. elegans. Basal bodies (BBs) and ciliary axonemes are marked with tdTomato-tagged XBX-1, a component of the ciliary dynein. Schematics illustrate the position of TCTN-1 at the transition zone with respect to the basal body and axoneme. Scale bar, 5 um.

Picture from Yee LE et al. (2015) PLoS Genet "Conserved Genetic Interactions between Ciliopathy Complexes Cooperatively ...."

Fig 1. The C. elegans ortholog of Tectonic1 is a transition zone component. (A) Schematic of the X-box sequence and ok3021 allele of C. elegans tctn-1.(B) GFP-tagged TCTN-1 localizes specifically to the transition zones (TZs) in head (amphid) and tail (phasmid) cilia of C. elegans. Basal bodies (BBs) and ciliary axonemes are marked with tdTomato-tagged XBX-1, a component of the ciliary dynein. Schematics illustrate the position of TCTN-1 at the transition zone with respect to the basal body and axoneme. Scale bar, 5 um.

Picture from Kim JS et al. (2010) Development "C. elegans STRADalpha and SAD cooperatively regulate neuronal polarity and ...."

Fig. 3. Pseudokinase STRD-1 functions cell- autonomously in neurons. (A)The expression of a Pstrd-1-nls::gfp reporter in the pharynx, excretory canal and the entire nervous system. (B)Cell-autonomous rescue of the neuronal polarity defect in strd-1 mutants. The VD polarity defect in strd-1(rr91) mutant animals was fully rescued by the expression of STRD-1 solely in GABAergic neurons under Punc-25. STRD-1(D175A) also fully rescued the VD polarity defect. Values are means ± s.d.; ***, P<0.001; N.S., not significant, Wilcoxon rank- sum test; n≥10 per strain. (C)Cell-autonomous rescue of strd-1 synaptic organization defect. strd-1(ok2283) mutants (i) expressing STRD-1 (ii) or STRD-1(D175A) (iii) in GABAergic neurons showed rescued juIs1 morphology. Distribution of the juIs1 punctum widths showed significantly reduced kurtoses in the rescued animals (P<0.001, strd-1 versus either STRD-1- expressing lines, t-test; n>80 per strain). x-axis, punctum width in pixels; y-axis, density in arbitrary units. (D)Sub-cellular localization of GFP-tagged, functional STRD-1 in GABAergic neurons. GFP::STRD-1 localized to the cytoplasm and the nucleus as well as along both nerve cords. Scale bars: 5um.

Picture from Steger KA et al. (2004) Genetics "The GAR-3 muscarinic receptor cooperates with calcium signals to regulate ...."

(B) A gar-3 reporter is expressed in neurons of the extrapharyngeal nervous system; in the pharyngeal muscles of the metacorpus, isthmus, and terminal bulb; and in pharyngeal neuron I3 (obscured by muscle staining). Bar, 50 um.

Picture from Jiu Y et al. (2012) PLoS One "Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate ...."

Figure S1 Confocal image of adult hermaphrodites expressing Psec-6::GFP under the 2065 bp promoter (Ex[Psec-6::GFP; pRF4]). Left is anterior. Scale bar, 100 um.

Picture from Lockwood C et al. (2008) Curr Biol "The C. elegans zonula occludens ortholog cooperates with the cadherin complex to ...."

Figure S2. ZOO-1 colocalizes with actin at cell borders during morphogenesis. Confocal images of embryos stained for ZOO-1 (A,D,G), F-actin (B,E,H), and the merge (C,F). (A-C) Embryo beginning ventral enclosure. (D-F) Embryo at the 1.25-stage of elongation has increased ZOO-1 at cell borders. (G, H) Embryo at the 2-fold stage of elongation has robust junctional ZOO-1. Insets in G and H are enlargements of the boxed regions. Scale bar, 10 um.

Picture from Lockwood C et al. (2008) Curr Biol "The C. elegans zonula occludens ortholog cooperates with the cadherin complex to ...."

Figure S3. ZOO-1 colocalizes with the cadherin complex in the epidermis. (A-C) Confocal images showing a comma stage embryo stained for ZOO-1 (A) that is also expressing JAC-1/p120-catenin::GFP (B). The merged image is shown in C (ZOO-1, red; JAC-1, green). C' shows a projected region of a junction in sagittal profile enlarged 3x. (D-F) Confocal images of a 1.5-fold stage embryo stained for ZOO-1 (D) and AJM-1 (E). The merged image is shown in F (ZOO-1, red; AJM-1, green). F' shows a projected region of a junction in sagittal profile enlarged 3X. Scale bar, 10 um. Quantitative colocalization analysis confirms that ZOO-1 colocalizes with the cadherin complex. For JAC-1, r2 = 0.72 ± 0.05, mean ± SEM, n = 3, whereas for AJM-1, r2 = 0.39 ± 0.05, n = 3 (significantly different from JAC-1, p < 0.004, Fisher's exact test).

Picture from Jiu Y et al. (2012) PLoS One "Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate ...."

Figure 1. Expression patterns of exocyst subunit exoc-7 in C. elegans. Confocal images of adult hermaphrodites expressing Pexoc-7::GFP under the 2954 bp promoter (Ex[Pexoc-7::GFP; pRF4]).