Picture from Colbert HA et al. (1997) J Neurosci "OSM-9, a novel protein with structural similarity to channels, is required for ...."

D, A lateral view of the head of a transgenic animal expressing theosm-9:: GFP5 fusion gene. GFP expression is present in the OLQ and IL2 sensory neurons and in the AWA, AWC, ASE, ADF, ASG, ASH, ASI, ASJ, ASK, and ADL amphid chemosensory neurons. In more posterior parts of the animal, the FLP, PVD, PHA, and PHB sensory neurons, the ventral uterine cells, and the rectal gland cells also express osm-9:: GFP5.

Picture from Colbert HA et al. (1997) J Neurosci "OSM-9, a novel protein with structural similarity to channels, is required for ...."

C, A lateral view of the head of a transgenic animal expressing the osm-9:: GFP3 fusion gene, which encodes a GFP-tagged OSM-9 protein. GFP is localized to the OLQ and AWA cilia at the tip of the nose (arrows), but little GFP is present in the cell bodies, axons, or dendrites. ADL expression is weaker or absent in these lines. The fusion gene deletes the last 105 amino acids of OSM-9; when GFP is fused to the last amino acid of OSM-9 (osm-9:: GFP4), a similar but fainter expression pattern is observed.

Picture from Colbert HA et al. (1997) J Neurosci "OSM-9, a novel protein with structural similarity to channels, is required for ...."

B, A lateral view of the head of a transgenic animal expressing the osm-9:: GFP2 fusion gene. GFP is expressed in the four OLQ mechanosensory neurons and in the AWA and ADL amphid chemosensory neurons. All neurons are bilaterally symmetric, and only those on the left side are visible in this focal plane. The fusion protein is present at high levels at the base of the OLQ cilia, but it is only weakly expressed in the cilia themselves.

Picture from Colbert HA et al. (1997) J Neurosci "OSM-9, a novel protein with structural similarity to channels, is required for ...."

Figure 5. osm-9:: GFP fusion genes are expressed in a subset of sensory neurons. A,osm-9:: GFP fusion genes. Three regions that regulate GFP expression in particular cell types are indicated. Allosm-9:: GFP fusion genes were examined in at least three independent transgenic lines. B, A lateral view of the head of a transgenic animal expressing theosm-9:: GFP2 fusion gene. GFP is expressed in the four OLQ mechanosensory neurons and in the AWA and ADL amphid chemosensory neurons. All neurons are bilaterally symmetric, and only those on the left side are visible in this focal plane. The fusion protein is present at high levels at the base of the OLQ cilia, but it is only weakly expressed in the cilia themselves.C, A lateral view of the head of a transgenic animal expressing the osm-9:: GFP3 fusion gene, which encodes a GFP-tagged OSM-9 protein. GFP is localized to the OLQ and AWA cilia at the tip of the nose (arrows), but little GFP is present in the cell bodies, axons, or dendrites. ADL expression is weaker or absent in these lines. The fusion gene deletes the last 105 amino acids of OSM-9; when GFP is fused to the last amino acid of OSM-9 (osm-9:: GFP4), a similar but fainter expression pattern is observed. D, A lateral view of the head of a transgenic animal expressing theosm-9:: GFP5 fusion gene. GFP expression is present in the OLQ and IL2 sensory neurons and in the AWA, AWC, ASE, ADF, ASG, ASH, ASI, ASJ, ASK, and ADL amphid chemosensory neurons. In more posterior parts of the animal, the FLP, PVD, PHA, and PHB sensory neurons, the ventral uterine cells, and the rectal gland cells also express osm-9:: GFP5.

Picture from Liu P et al. (2013) PLoS One "Six innexins contribute to electrical coupling of C. elegans body-wall ...."

Subcellular localization of INX-18::HA. INX-18::HA mainly showed punctate localization at muscle intercellular junctions.

Picture from Corrionero A et al. (2018) Curr Biol "A C9orf72 ALS/FTD Ortholog Acts in Endolysosomal Degradation and Lysosomal ...."

(F) Anti-HA tag immunofluorescence of proliferative (left) and comma-stage (right) embryos expressing the reporter nIs759[Palfa-1::alfa-1 gDNA::HA::alfa-1 3'UTR]showing ALFA-1::HA cytoplasmic localization. Scale bar, 10 mm.

Picture from Kolundzic E et al. (2018) Dev Cell "FACT Sets a Barrier for Cell Fate Reprogramming in Caenorhabditis elegans and ...."

(F) hmg-3 with HA and (G) hmg-4 with HA. Dashed lines indicate the outline of the animals. Scale bars represent 20 um.

Picture from Bergwell, Mary et al. MicroPubl Biol

Figure 1 Generation of IYR001 strain containing sas-6(luv1[sas-6::ha]), and its localization in the early C. elegans embryo A) Left panel: Cartoon depicting nucleotide sequences flanking the ha-tag sequence in the designed repair template to generate the sas-6::ha Red text- Silent mutations introduced in the repair template; Teal highlight- part of homology arms. Right panel: Cartoon depicting DNA fragment sizes expected upon PCR followed by NdeI digestion for wild-type and sas-6::ha edited worms. B) Agarose gel electrophoresis of NdeI digested sas-6 PCR products to screen for homozygous edited sas-6(luv1[sas-6::ha]) worms. L: 1Kb DNA ladder, Yellow stars: sas-6::ha homozygous worms. C) Immunostaining with an anti-HA antibody shows localization of SAS-6::HA to the centrosomes (insets) and in the cytoplasm in 1-cell C. elegans embryos during different stages of mitosis. Microtubules (red), SAS-6::HA (green) and DNA (blue).

Picture from Kolundzic E et al. (2018) Dev Cell "FACT Sets a Barrier for Cell Fate Reprogramming in Caenorhabditis elegans and ...."

(G) hmg-4 with HA. Dashed lines indicate the outline of the animals. Scale bars represent 20 um.

Picture from Griffitts JS et al. (2003) J Biol Chem "Resistance to a bacterial toxin is mediated by removal of a conserved ...."

BRE-3 is in cytoplasmic puncta. A, double staining (HA is in red; 4',6-diamidino-2-phenylindole (DAPI) is in blue) of HA-tagged BRE-3 driven by the cpr-1 promoter. BRE-3 localizes to cytoplasmic puncta and is not seen at the plasma membrane.