Picture from Colavita A et al. (1998) Science "Pioneer axon guidance by UNC-129, a C. elegans TGF-beta."

Figure 2. (left). Expression pattern and cell nonautonomous activity of unc-129. (A) Comparison ofunc-129::gfp transcriptional reporters used in this study with unc-129 rescuing genomic DNA. (Bto E) unc-129::gfp expression pattern at ~280 min (B), ~430 min (C), ~550 min (D) postcleavage, and in LI larvae (E). Expression in dorsal body wall muscle [arrowheads in (D) and (E)] is observed before DA and DB expression. GFP expression vectors used in (B) to (D) contain a nuclear localization signal. (F and G) unc129::gfpreporter derivatives expressed predominantly in DA and DB motorneurons (F) (arrow indicates dorsal nerve cord) and dorsal body wall muscle in L1 larvae (G). (H) unc-129 expression from the dorsal muscle-specific promoter is sufficient to completely rescue theunc-129 phenotype. Restriction sites as in Fig. 1. Bar, 10 um.

Picture from Colavita A et al. (2003) Science "A Neurexin-related protein, BAM-2, terminates axonal branches in C. ...."

Figure 3. Expression and cell-nonautonomous activity of bam-2. (A) Expression and rescue ability of bam-2 promoter derivatives driving a full-length bam-2 cDNA::gfp fusion gene. bam-2 expression from the VulF vulval cell rescues bam-2 VC neuron-branching defects. (B to D) Expression pattern of bam-2 promoter derivatives (ventral views). (B) bam-2 promoter (~600 bp) exhibits activity in a restricted subset of neurons in the ventral nerve cord and head and tail ganglia. (C) bam-2 upstream sequence (~5 kb) exhibits additional promoter activity in cells of the somatic gonad. The position of the vulva in (B) and (C) is marked by arrowheads. (D) A fusion of a ~2.4-kb distal bam-2 upstream sequence and the 600-bp proximal element exhibits promoter activity in additional longitudinal PLN axon tracts (asterisks) and in the VulF vulval cell. Inset shows an enlarged view of VulF. (E and F) bam-2 is expressed from mid-L4 in VulF (open arrowheads). Lateral views show overlay of VulF GFP fluorescence (delta-xbaIp:bam-2::gfp) (E) on a differential interference contrast image of the same L4 worm (F). (G and H) delta-xbaIp:bam-2::gfp expression in the toroidal-shaped VulF cell [(G), ventral view] and in an adult worm [(H), lateral view]. All images except (E) and (F) are flattened confocal Z-series. Anterior is to the left. Scale bars, 10 um.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red).