Picture from Cohen, Sarah M et al. MicroPubl Biol

Figure 1. Transcriptional response to ascarosides of C. elegans in early L1 stage: (a) The top ten up-regulated and (b) down-regulated genes in N2 (wild-type) L1 larvae. (c) The top ten up-regulated and (d) down-regulated genes in daf-22 mutant L1 larvae. (e) A heatmap of the most significant genes at p = 0.001 with annotated biological functions, derived from WormBase Gene Ontology annotations, in the N2 and daf-22 samples. A full list of the significant genes at p = 0.001 can be found in the Extended Data.

Picture from Cohen, Sarah M et al. MicroPubl Biol

Figure 1: (A) Wildtype AF16 worms as compared with (B) Cbr-dpy-10, which shows the Dumpy phenotype.

Picture from Cohen M et al. (2009) Cell Metab "Coordinated regulation of foraging and metabolism in C. elegans by RFamide ...."

Figure 4. The flp-18 Receptors npr-4 and npr-5 Act in Multiple Tissues to Regulate Fat (A-D) Expression of npr-4p::RFP (red) and DiO (3,30 -dioctadecyloxacarbocyanine, perchlorate)- filled neurons (green). Shown are confocal projec- tions: lateral view of head neurons (A) and ventral view of tail neurons (B; arrowheads indicating PHA and PHB and arrow indicating PQR). Also shown are npr-4 expression in coelomocytes (indi- cated by arrow) and in the intestine (C; confocal image, lateral view) and npr-4 expression in the intestine and the BDU neuron, indicated by an arrow (D; confocal image, lateral view).(E-H) Expression of npr-5p::RFP (red) and DiO filled neurons (green). Shown are confocal projec- tions: lateral view of head neurons (E) and ventral view of tail neurons (F; arrowheads indicating PHA and PHB). Also shown are npr-5 expression in head and neck muscles (G; confocal image, lateral view) and npr-5 expression in body wall muscles. (H; confocal image, lateral view). Scale bars are 10 mm.(I) Quantification of Nile Red fluorescence of L4 animals. Expression of npr-5 selectively, in a group of ciliated neurons, rescues the fat phenotype of npr-5. Ns: N2 = 157, flp-18(db99) = 235, npr- 5(ok1583) = 141, npr-5(ok1583) Ex[tax-4p::npr-5] = 121, npr-5(ok1583) Ex[odr-4p::npr-5] = 53.(J) Quantification of Nile Red fluorescence of L4 animals. Ns: N2 = 75, npr-4 = 157, npr-4(tm1782) Ex[elt-2p::npr-4] = 129. Error bars indicate SEM.

Picture from Cohen M et al. (2002) J Struct Biol "Transmission electron microscope studies of the nuclear envelope in ...."

Figure 3. Immunogold TEM labeling of lamin in C. elegans embryos. Rabbit antibodies against the rod and tail regions of Ce-lamin were used as primary antibodies and 12 nm gold-conjugated goatanti-rabbit IgG secondary antibodies were used to localize Ce-lamin in C. elegans embryos. The positions of the gold particles near the inner nuclear membrane and in the nuclear interior are marked by arrows. Nucleus, Nu; Cytoplasm, Cyt.

Picture from Cohen M et al. (2009) Cell Metab "Coordinated regulation of foraging and metabolism in C. elegans by RFamide ...."

(A-D) Expression of npr-4p::RFP (red) and DiO (3,30 -dioctadecyloxacarbocyanine, perchlorate)- filled neurons (green). Shown are confocal projec- tions: lateral view of head neurons (A) and ventral view of tail neurons (B; arrowheads indicating PHA and PHB and arrow indicating PQR). Also shown are npr-4 expression in coelomocytes (indicated by arrow) and in the intestine (C; confocal image, lateral view) and npr-4 expression in the intestine and the BDU neuron, indicated by an arrow (D; confocal image, lateral view). Scale bars are 10 um.

Picture from Cohen M et al. (2009) Cell Metab "Coordinated regulation of foraging and metabolism in C. elegans by RFamide ...."

(E-H) Expression of npr-5p::RFP (red) and DiO filled neurons (green). Shown are confocal projections: lateral view of head neurons (E) and ventral view of tail neurons (F; arrowheads indicating PHA and PHB). Also shown are npr-5 expression in head and neck muscles (G; confocal image, lateral view) and npr-5 expression in body wall muscles. (H; confocal image, lateral view). Scale bars are 10 um.

Picture from Lall S et al. (2005) Mol Biol Cell "Caenorhabditis elegans decapping proteins: localization and functional ...."

Figure 1. C. elegans Dcp2 is found in cytoplasmic bodies from early embryogenesis. (A) Western blots using the anti-Dcp2 antisera on C. elegans mixed stage embryo extract reveal a dominant band close to 90 kDa. A second lower band was observed upon Western blotting of C. elegans embryonic extracts with two different antisera raised to Dcp2 protein. This lower band may represent a breakdown product of Dcp2, as a similar but slower migrating band is frequently observed upon purification of recombinant Dcp2 (Cohen et al., 2005). (B) dcp2 RNAi depletes the particular staining, indicating that these foci are due to specific staining of Dcp2. Punctate Dcp2 staining is observed in embryos treated with dsluciferase RNA (top panel), but is absent in the majority of embryos treated with dsdcp2 RNA (bottom panel). In addition the disappearance of these particles upon dsdcp2 treatment suggests that this RNAi treatment diminishes levels of the protein. This shows that the immunostaining is specific and that Dcp2 RNAi is depleting the protein recognized by the antibody. Embryos shown are at the division following the four-cell stage when particles are easily visualized. (C) Immunolocalization of Dcp2 shows that punctate staining is first observed before the pronuclei fuse (top row). Cytoplasmic Dcp2 foci are seen in both the somatic cells and the germ line lineage in the 2-cell (second row), and 8-cell (third row) embryo. The fourth row shows a closer view of punctate Dcp2 staining in an embryonic cell in C. The intensity of foci typically appears to increase during the initial divisions in development up to the 8-cell stage, with strong labeling of somatic foci then observable throughout embryogenesis. This is consistent with an increase in Dcp2 foci as maternal transcripts are turned over or as zygotic transcription initiates. (D) CGH-1 and Dcp2 completely colocalize in cytoplasmic foci in early embryos, with CGH-1 becoming difficult to detect subsequently in development.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.