Fig. 4. Expression of GFP-tagged CeCRMP/DHP-1(A) and CeCRMP/DHP-2 (B) at the 3-fold embryo (egg), L1-L2 larva, L3-L4 larva and adult stages. Nomarski images (N) and fluorescent images (F). Scale bar, 50 um. The expression of pCeCRMP/DHP-1::NLS::GFP was observed at the 3-fold embryo stage and at the L1-L2 larva stage, but not at the 2-fold embryo stage (data not shown) or at the L3-L4 larval stage (A). The GFP fluorescence reappeared in the adult body hypodermal cells and pharynx (A). In an enlarged view of L1-L2 larva (A-a,b,c), the expression in the nuclei of body hypodermal cells, including
hyp7, and the expression in the hemidesmosomes are clearly shown (A-b). In the head region, fluorescence of a single cell was detected between the pharynx and ring neuropil (A-c). The expression of pCeCRMP/DHP-2::NLS::GFP was observed in body wall muscle cells at each stage (B). Expression of pCeCRMP/DHP-2::GFP (CeCRMP/DHP-2 without NLS) was observed in the whole cytoplasm of body wall muscle cells (B-b). In an enlarged view, the expression was confirmed in the cytoplasm of a single muscle cell (B-c).