Picture from Snow MI et al. (2000) Biochim Biophys Acta "Structure and expression of daf-12: a nuclear hormone receptor with three ...."

Fig. 4. Developmental Northern analysis. (A) One Wg of the twice-selected poly(A)+ RNA was fractionated on a 1% formaldehyde contain- ing agarose gel, transferred to a nylon membrane and was probed with 32P-labeled anti-sense RNA from a daf-12 B isoform clone template. The lanes are labeled with the developmental stage of the animals from which RNA was isolated. (B) To access loading, the membrane was probed with a cDNA clone, yk104e5, which encodes ribosomal protein 27 fused to ubiquitin. Numbers depicted on the right side of figure indicate size of RNA in kilobases derived from molecular mass markers. (C) The daf-12 and yk104e5 expression levels were quantified with a phosphoimager. The y-axis represents the normalized volume of pixels detected by the phosphoimager.