Figure 2. NRF-5 Is a Secretory Protein from Body Wall Muscle Cells and Clusters around Apoptotic Cells (A-F' ) Differential interference contrast (DIC) and fluorescent images of WT (A-B', D-E') or
ced-3(
n717) embryos (C, C', F, F ' ) that carry PhspNRF-5::mCHERRY (A, A', C, C' ), PhspNRF- 5(21-551)::mCHERRY (B, B' ), Pced-1ssGFP:: mNRF-5 (D, D', F, F'), or Pced-1GFP::mNRF-5 (E, E'). Arrows indicate apoptotic cells, and arrowheads point to accumulated fluorescent proteins in the extraembryonic cavity. Cell corpses indicated by yellow arrows are shown at 33 magnification in the insets. (G-H''') DIC and fluorescent images of WT animals transgenic for both Pnrf-5GFP and Pmyo-3 mCHERRY. Scale bars in all panels represent 5 um.