Picture from Loucks CM et al. (2016) Mol Biol Cell "PACRG, a Protein Linked to Ciliary Motility, Mediates Cellular ...."

(B) Localization of EFHC-1::GFP to the four OLQ and the four CEP cilia of C. elegans. Basal body regions (BB) areindicated by orange ovals, cilia by green lines, and dendrites by gray lines. Scale bar, 5 um.

Picture from Cevik S et al. (2013) PLoS Genet "Active transport and diffusion barriers restrict Joubert Syndrome-associated ...."

(B) Co-expression of ARL-13::GFP with CHE-13/IFT57::mCherry, or ARL-13::tdTomato with either OSM-6::GFP or MKSR-1/B9D1::GFP, show that C. elegans ARL-13 is excluded from the transition zone (TZ). DS; distal segment. MS; middle segment. BB; basal body. PCMC; periciliary membrane compartment. Bars; 1 mm.

Picture from Li C et al. (2016) PLoS Biol "MKS5 and CEP290 Dependent Assembly Pathway of the Ciliary Transition ...."

(A) C. elegans CEP-290 (Y47G6A.17) tagged with GFP (green) is expressed only in ciliated sensory neurons (including amphids and phasmids) and isspecifically enriched at the ciliary transition zone (TZ). tdTomato-tagged XBX-1 (red) is an IFT protein that marks the basal body (BB) and axoneme. Scale bars in panels A, C, and D are 4 um.

Picture from Kazatskaya A et al. (2017) Genetics "Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the ...."

(B) Representative images of the indicated GFP-tagged MAPK-15 reporters in wild-type worms. Constructs (shown in schematicsat right) derived from mapk-15 genomic sequences (red) under the control of mapk-15 upstream regulatory promoter sequences(Prom; orange). Strains expressing the mapk-15a::gfp DNA transgene at low levels generated by injecting worms with a low DNAconcentration (0.2 ng/ul). BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tailimages). Scale bars: 3 um (head images) and 5 um (tail images).(C) Representative images of localization of GFP::MAPK-15c expressed under its endogenous promoter in mapk-15(gk1234)animals. BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tail images). Scale bars:5 um.

Picture from Li C et al. (2016) PLoS Biol "MKS5 and CEP290 Dependent Assembly Pathway of the Ciliary Transition ...."

(B) C. eleganstmem-218 (T23E7.5) is expressed specifically in ciliated sensory neurons and its encoded product (TMEM-218) tagged with green fluorescent protein (GFP;green) localises to the ciliary transition zone (TZ) at amphid (head) and phasmid (tail) cilia found at the distal ends of dendrites. The tdTomato-tagged intraflagellar transport (IFT) protein XBX-1 (red) marks basal bodies (BB) and ciliary axonemes. Scale bar, 4 um.

Picture from Timbers TA et al. (2016) PLoS Genet "Accelerating Gene Discovery by Phenotyping Whole-Genome Sequenced ...."

B) ADL cilia correctly enter the amphid socket (AMso) cells in bgnt-1.1 mutants. ADL cilia are labelled with Psrh-220::IFT-20::tdTomato. IFT-20(IFT20) localises to cilia basal bodies (bb) and axonemes. The srh-220 promoter drives expression primarily in ADL neurons. The amphid socket (AMso) cells are labelled with cytoplasmic GFP driven by an amphid socket specific promoter, grd-15 (Hunt-Newbury et al. 2007).

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

(Ba-Bd) INA-1::GFP and SRC-1::mCherry are colocalized to the pseudopods extending around an apoptotic cell. INA-1::GFP (Ba), SRC-1::mCherry (Bb), merged INA-1::GFP and SRC-1::mCherry (Bc), and DIC (Bd) images of the same embryo coexpressing Pced-1ina-1::gfp and Pced-1src-1::mcherry are shown. Apoptotic cells are indicated by arrows and are magnified in the insets. Scale bar in (Ba) represents 5 um.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Figure 6. Nucleolar localization of H1.X (A) shows a Nomarski micrograph of the nuclear region of a polyploid gut cell in a hermaphrodite C. elegans with the nucleolus and its substructures visualized by differential interference contrast. (B) H1.X detected with the antibody anti-H1.X-101 localizes to the nucleolus; (C) corresponds to DAPI DNA staining; and (D) shows the indirect immunfluorescence detection of the nucleolar protein fibrillarin with the specific monoclonal antibody P2G3 (from M. Christensen). Bar, 10 um.

Picture from Dittman JS et al. (2006) Proc Natl Acad Sci U S A "Factors regulating the abundance and localization of synaptobrevin in the ...."

Fig. 2. Imaging synaptobrevin in intact animals. (Aa) The N-terminal tag places GFP (NGFP-SNB) in the cytoplasm. (Ab) Motor neurons expressing a C-terminal pHluorin-tagged synaptobrevin (SpH) that places the fluorophore in the vesicle lumen. (Scale bars, 5 um.) (Ba) Expression of SpH in the ventral cord. Neuromuscular junctions are located on the bottom cord (*). (Bb) Expression of UNC-10 RIM1::RFP in the ventral cord. (Bc) Colocalization of SpH (green) and UNC-10 (red). (C) A line scan of SpH in a wild-type animal. Peaks (red arrowheads) and baseline (dashed line) were located by using automated software, as described in Supporting Methods.

Picture from Church DL et al. (2003) Genetics "The promotion of gonadal cell divisions by the Caenorhabditis elegans TRPM ...."

Figure 5. Expression of GEM- 4::GFP in L1 larvae. (A and B) DIC and GFP fluorescence images of an early L1 stage animal of genotype gon-2(q388); gem-4(dx77); dxEx15[gem-4::GFP; pRF4] raised at 15C [permissive temperature for gon-2(q388)]. (C and D) DIC and GFP fluorescence images of an early L1 stage animal of genotype gon-2(q388); dxEx15 [gem-4::GFP; pRF4] raised at 25C [restrictive temperature for gon-2(q388)]. gem-4:: GFP is also localized to the intestinal brush border (bb). The granular/globular fluorescence in B and D is due to autofluorescence of gut granules. Bar, 10 um.