Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

B) A confocal image of animals expressing endogenous GOT-1::GFP from the knock-in allele (green), and the Pnmr-1-RFP reporter (red).Robust expression of UBR-1 and GOT-1 was present at the pharynx; neuronal UBR-1::GFPand GOT-1::GFP signals were present in the AVA, AVE and RIM premotor interneurons(denoted), and other unidentified neurons (not shown). Scale bar, 5 um.(TIF)

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

B) GOT-1 is ubiquitously expressed in all somatic tissues. Scale bar 10um.

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

Fig 2. A critical requirement of UBR-1 in premotor interneurons during reversals. A) The UBR-1::GFP reporter was expressed in a subset of neurons including thepremotor interneurons of the reversal circuit (Premotor INs), the pharyngeal muscles, the body wall muscles, and hypodermal seam cells. Scale bar 10um. B) Reducedbending in ubr-1 mutants (grey line) was rescued by restoring UBR-1 expression in neurons (red line), but not in muscles (blue line). C) Reduced bending in ubr-1mutants (grey line) was robustly rescued by restoring UBR-1 expression in glutamate-receptor-expressing, premotor interneurons (INs), not in cholinergic or GABAergicmotor neurons (MNs). D) Among the premotor interneurons (INs), a robust rescue required UBR-1 expression in AVE and RIM (red line). P<0.05, P<0.01, P<0.001 by Two-way RM ANOVA. Data are represented as mean +- SEM.

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) The UBR-1::GFP reporter was expressed in a subset of neurons including thepremotor interneurons of the reversal circuit (Premotor INs), the pharyngeal muscles, the body wall muscles, and hypodermal seam cells. Scale bar 10um.

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red).

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

S2 Fig. UBR-1 and GOT-1 are expressed in AVA, AVE and RIM. A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red). B) A confocal image of animals expressing endogenous GOT-1::GFP from the knock-in allele (green), and the Pnmr-1-RFP reporter (red).Robust expression of UBR-1 and GOT-1 was present at the pharynx; neuronal UBR-1::GFPand GOT-1::GFP signals were present in the AVA, AVE and RIM premotor interneurons(denoted), and other unidentified neurons (not shown). Scale bar, 5 um.(TIF)

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

Fig 4. Removing GOT-1 transaminase restores bending in ubr-1 mutants. A) A diagram depicting the structure of GOT-1 and the molecularlesion of the hp731 allele. B) GOT-1 is ubiquitously expressed in all somatic tissues. Scale bar 10um. C) Mutations in got-1 suppress motor neurondefects in ubr-1. D) Restoring GOT-1 in premotor INs including AVE and RIM (blue line) in ubr-1; got-1 reverted curvature to that of ubr-1. P<0.05, P<0.01, P<0.001 by the Two-way RM ANOVA test. Data are represented as mean +- SEM.

Picture from Inoue T et al. (2004) Proc Natl Acad Sci U S A "Type II platelet-activating factor-acetylhydrolase is essential for ...."

(I and J) paf-2::GFP expression in embryos (lateral view, dorsal is up). Nomarski micrographs (I) and corresponding paf-2::GFP expression (J). paf-2 reporter gene constructs are expressed in epidermal cells (J, arrowheads) and intestinal cells (J, arrow) at the beginning of elongation. Anterior is to the left. (Bar, 10 um.)

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

(J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene.

Picture from Zhang D et al. (2012) J Cell Biol "RAB-6.2 and the retromer regulate glutamate receptor recycling through a ...."

Figure 2. RAB-6.2 is broadly expressed in multiple tissues. (A-I) Fluorescence from Prab-6.2::GFP in body wall muscles (A), pharyngeal muscle and head neurons (B), hypodermal cells (C), the seam cells (D), intestinal epithelia (E), coelomocytes (F), vulval epithelia (G), the distal tip cells (H), and the vulval muscles and ventral cord dendrites (I). (J-J'') Fluorescence from Prab-6.2::GFP (J) and Pglr-1::mRFP (J') with images merged (J''). Coexpressing neurons are outlined. Bars, 5 um.