Picture from Cheng H et al. (2008) Curr Biol "Regulated trafficking of the MSP/Eph receptor during oocyte meiotic maturation ...."

Cortical views of VAB-1::GFP expression and RAN-1 staining in dissected and fixed gonads from female (upper panel) and hermaphrodite animals (lower panel); insets in the upper left are magnified views of the indicated regions. Scale bars represent 10 um.

Picture from Cheng H et al. (2008) Curr Biol "Regulated trafficking of the MSP/Eph receptor during oocyte meiotic maturation ...."

Figure 5. DAB-1 and RAN-1 Bind to the VAB-1 Intracellular Domain.(A) In vitro interaction of GST-DAB-1(53-546) and MBP-VAB-1-ICD. Complexes were isolated with glutathione sepharose. MBP and GST served as negative controls. Western blots were probed with anti-MBP (top panel) and anti-GST (middle and bottom panels). The bottom panel shows the position of GST and breakdown products of GST-DAB-1(53-546).(B) Biochemical purification of VAB-1-ICD-interacting proteins. A flow chart depicts the purification strategy starting with 30 ml of packed adult hermaphrodites. The bottom panel shows the sequence of RAN-1 tryptic peptides that were retained on the MBP-VAB-1-ICD column at high stringency but were absent from the empty column control.(C) In vitro interaction of 6His-SUMO-RAN-1 and MBP-VAB-1-ICD. Complexes were isolated with amylose sepharose. 6His-SUMO and MBP served as negative controls. Western blots were probed with anti-6His (top panel) and anti-MBP (middle and bottom panels). The bottom panel shows the position of MBP and breakdown products of MBP-VAB-1-ICD.(D) Cortical views of VAB-1::GFP expression and RAN-1 staining in dissected and fixed gonads from female (upper panel) and hermaphrodite animals (lower panel); insets in the upper left are magnified views of the indicated regions. Scale bars represent 10 um.

Picture from Dong X et al. (2013) Cell "An extracellular adhesion molecule complex patterns dendritic branching and ...."

(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(g-h) vrk-1::gfp was expressed in several cells, which included head neurons (g) and phasmids (h).

Picture from Fisher J et al. (2007) PLoS Comput Biol "Predictive modeling of signaling crosstalk during C. elegans vulval ...."

As examples for the time-course analysis, a mid-L2 larva at +22 h (A') and a late L2 larva at +28 h (B') are shown.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(H) Pgrl-27::GFP is expressed in arcade cells.

Picture from Yu S et al. (1997) Proc Natl Acad Sci U S A "Guanylyl cyclase expression in specific sensory neurons: a new family of ...."

(H 1-3) Expression pattern of gcy-32::GFP.

Picture from Fisher J et al. (2007) PLoS Comput Biol "Predictive modeling of signaling crosstalk during C. elegans vulval ...."

As examples for the time-course analysis, a mid-L2 larva at +22 h (A'') and a late L2 larva at +28 h (B'') are shown.

Picture from Cuppen E et al. (2003) Comp Funct Genomics "Proteins interacting with Caenorhabditis elegans Galpha subunits."

Expression pattern of T22A3.3 (H) as determined by promoter-GFP reporter constructs. (H) Expression of T22A3.3 is seen in a set of neurons in the head, including AWB.

Picture from Tepper RG et al. (2013) Cell "PQM-1 complements DAF-16 as a key transcriptional regulator of DAF-2-mediated ...."

(F-H) DAF-16 becomes more cytoplasmic with age, and nuclear localization of PQM-1::GFP decreases significantly with age. Data are represented as mean +/- SEM (G and H).