(B) Wild-type perinuclear germline localization of DRH-3,(left, yellow) (DNA, center, blue).(C) DRH-3 (left, green) colocalizes with the P granule component, PGL-1 (center, red; DNA, blue).(D) DRH-3 (left, yellow) remains localized to P granules in the embryonic P cell lineage (dashed circles; DNA, center, blue).
A). The sgk-1 isoform A promoter drives strong expression inthe intestine, but not in neurons. Left, bright field; center, Venus; right, merged. Scale bar:100 um.
Figure 6. UNC-83 co-localization with Lamin and UNC-84. (A) Co-localization of UNC-83 (left panel) and Lamin (center panel) shown merged together (right panel) with UNC-83 pseudocolored red and Lamin in green. (B) Co-localization of UNC-83 (left panel) and UNC-84::GFP (center panel) shown merged together (right panel) with UNC-83 pseudocolored red and UNC-84::GFP green. Scale bar: 2 um.
(C) An ets-5::mCherry translational reporter shows expression in BAG sensory neurons. Left, DIC image; center, epifluorescence image; right, overlay. Anterior is left; dorsal is up. Arrowheads indicate BAG neurons.
(G) Representative of three center images of confocal photomicrographs of animals expressing egl-20p::mcherry or egl-20p::egl-20p::mCherry in combinationwith rgef-1p::Q40::yfp. Scale bar represents 50 um.
Localization of LIM-8 is shown together with antibodies to markers for dense bodies (alpha-actinin) and M-lines (UNC-89). The left column shows LIM-8 localization. The center column shows dense body and M-line marker localization. The right column shows merged images of left and center columns. Rabbit antibodies were visualized by anti-rabbit IgG conjugated with Alexa 488, and mouse antibodies were visualized by anti-mouse IgG conjugated with Alexa 647.
Localization of LIM-9 in adult body wall muscle cells. Localization of LIM-9 is shown together with antibodies to markers for dense bodies (alpha-actinin) and M-lines (UNC-89). The left column shows LIM-9 localization. The center column shows dense body and M-line marker localization. The right column shows merged images of left and center columns. Rabbit antibodies were visualized by anti-rabbit IgG conjugated with Alexa 488, and mouse antibodies were visualized by anti-mouse IgG conjugated with Alexa 647. White bar, 10 um.