Figure 1. ZYX-1 regulates embryo transit through the spermatheca: (A) Schematic of ZYX-1a, with location of
zyx-1(
xb6) deletion indicated. Image made with BioRender. (B) Expression pattern for ZYX-1. ZYX-1::GFP is expressed in the pharynx, body wall muscles, and spermatheca. (C) Population assay of wild type animals and
zyx-1(
xb6). Spermathecae were scored for the presence or absence of an embryo (occupied or unoccupied), or presence of a fragment of an embryo (small piece occupied). The total number of unoccupied spermatheca were compared to the sum of all other phenotypes using the Fisher's exact t-test. N is the total number of spermathecae counted. (D) Schematic representation of a spermatheca undergoing an ovulation, with entry, dwell, and exit times indicated. Entry time (E), dwell time (F), and exit time (G) analysis of wild type animals and
zyx-1(
xb6) ovulations were compared using Fishers exact t-test. (H) Confocal images of wild type and
zyx-1(
xb6) worms expressing ACT-1::GFP were analyzed using Fibriltool (I) and compared using Fishers exact t-test. Stars designate statistical significance (** p<0.01).