Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Symmetric expression of DPY-22 in the T-cell division. Expression of dpy-22::GFP (A,B) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Symmetric expression of LET-19 in the T-cell division. Expression of let-19::GFP (C,D) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Lear SK et al. (2018) MicroPubl Biol "Immunofluorescence reveals neuron-specific promoter activity in ...."

In case of the pan-neuronal promoters, expression under cmk-1 promoter is observed in most, if not all neuronal nuclei, including in many head neurons (C top) and ventral nerve cord (VNC) neurons (C bottom).

Picture from Lu Q et al. (2011) Dev Cell "The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of ...."

Q-T. Expression of gfp::epg-6 in the head region (Q), body wall muscles (R), the tail region (S) and the ventral cord (T) at larval stages.

Picture from Akerib, Chantal C et al. MicroPubl Biol

Figure 1. Nature of the rad-54L(me139) mutation:A. Schematic of the rad-54.L gene structure taken as a screenshot from WormBase WS285, indicating the position of the 102 nt deletion in the me139 mutant. B. Sequence context of the me139 deletion. UTR and intron sequences are indicated in lower case, exon sequences in upper case. The nucleotides deleted in the me139 mutant are indicated by cyan highlighting, and potential cryptic splice donor sequences that could be used to generate modified proteins retaining key conserved protein domains are indicated as depicted on figure.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Figure 5. Symmetric expression of LET-19 and DPY-22 in the T-cell division. Expression of dpy-22::GFP (A,B) and let-19::GFP (C,D) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Etchberger JF et al. (2007) Genes Dev "The molecular signature and cis-regulatory architecture of a C. elegans ...."

Suppl. Fig.1: Expression pattern of genes coding for transcription factors and signaling genes from the SAGE library as assessed by gfp reporter gene technology. Reporter gene constructs were generated for genes from the SAGE library as shown. Green boxes indicate gfp coding sequences (rfp in the case of dop-3 and yfp in the case of hlh-10). If extrachromosomal arrays were generated, multiple lines were analyzed for each reporter construct, as indicated under each panel of micrographs. Each dataset of micrographs shows expression of the reporter construct under investigation in a high-magnification (400x) image, the overlap with a fluorescent marker for ASEL/ASER (in all except one case, the otIs151 transgene) and a low magnification (160x) overview of expression of the reporter construct under investigation throughout the whole worm. In the low magnification images, the asterisk (*) indicates expression of the gut-specific injection marker elt-2::gfp.

Picture from Lockhead D et al. (2016) Mol Biol Cell "The tubulin repertoire of C. elegans sensory neurons and its context-dependent ...."

(B) Coexpression of alpha-tubulin and beta-tubulin TagRFP-T protein fusions and uIs31 TRN::GFP transcriptional fusion. Left, uIs31(mec-17p::gfp) showing PLM. Center, TagRFP-T tubulin fusion protein. Right, composite image. TagRFP-T was false colored as magenta for enhanced visibility. Expression of isoforms inTRNs is seen as white in the composite overlap of uIs31 marker and TagRFP-T::Tubulin. Images are oriented so that the anterior of the animal is on the left and the ventral side is on the bottom. Scale bar, 50 um.

Picture from Dickinson DJ et al. (2018) MicroPubl Biol "SapTrap assembly of repair templates for Cas9-triggered homologous ...."

Figure 1. A) General organization of SapTrap-assembled constructs. Italics indicate the fragments that are assembled to build the final construct, and blue upper-case letters are the 3-nucleotide overlaps that enable ordered assembly. B) Efficiency of construct assembly (% correct clones) using SapTrap (Schwartz and Jorgensen 2016) or a ccdB/Gibson-based approach (Dickinson et al. 2015).

Picture from Weber KP et al. (2012) BMC Evol Biol "Analysis of C. elegans NR2E nuclear receptors defines three conserved clades and ...."

D. Expression of the nhr-239::gfp transgene in a two-fold embryo (EMB) and adult (Ad) hermaphrodites. Individual neuron pairs are identified by lower case letters: a, pair of pharyngeal neurons; b, pair of mid-lateral neurons or neuronal support cells; c, pair of dorsal neurons just posterior to the nerve ring with sensory dendritic processes (arrowhead).