Picture from Pierce SB et al. (2001) Genes Dev "Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the ...."

ins-1::GFP expression in amphid neurons (vertical arrow marks sensory processes) and pharyngeal neurons (diagonal arrow marks cell process).

Picture from Furuta T et al. (2018) MicroPubl Biol "GFP::PCN-1 does not reliably mark S phase in C. elegans adult germline progenitor ...."

Figure 1. The figure shows an adult dissected hermaphroditic germline oriented with distal tip cell (*) on the left. The germline is stained with anti-GFP antibody, to visualize GFP::PCN-1 (green), DAPI to visualize the DNA (white) and EdU to visualize S phase cells (red). The solid line marks the progenitor zone. Arrowhead marks an example of non-EdU incorporating cell, with nuclear GFP::PCN-1. Arrow marks an example of non-nuclear GFP::PCN-1 in metaphase cells. Scale bar: 50μm.

Picture from Phillips CM et al. (2005) Cell "HIM-8 binds to the X chromosome pairing center and mediates chromosome-specific ...."

Pachytene nuclei were stained with antibodies against HIM-8 (yellow) and anti-LMN-1 (red), which marks the nuclear lamina, or nuclear envelope. Scale bar represent 5 μm.

Picture from Barker TJ et al. (2023) MicroPubl Biol "Apical-basal polarity of the spectrin cytoskeleton in the C. elegans ...."

Figure 1. Apical-basal polarity of the spectrin cytoskeleton in the C elegans vulva:A, A') SPC-1/α-spectrin marks all vulva cell membranes; A' is a cartoon tracing of the 7 vulA-vulF rings. B, B') UNC-70/β-spectrin marks basolateral membranes. C, C') SMA-1/βH-spectrin marks apical cell membranes. B' and C' cartoons are drawn in comparison to A'. D) VAB-10a/spectraplakin marks apical vulva cell membranes. E, F) SMA-1/βH-spectrin is required for apical localization of SPC-1/α-spectrin (E) but not VAB-10a/spectraplakin (F). F' shows DIC channel, with disorganized tissue occluding the connection between the gonad and the dorsal apex of the vulva (cog phenotype, arrow). All images are single confocal Z-slices through the medial portion of the mid-L4 vulva tube and are representative of at least n=10 animals examined per genotype.

Picture from Lesch BJ et al. (2009) Genes Dev "Transcriptional regulation and stabilization of left-right neuronal identity ...."

(C) Nuclear localization of a NSY-7::GFP protein expressed in both AWCs under the odr-3 promoter. str-2::dsRed marks AWCON; note gain-of function 2-AWCON phenotype.

Picture from Fox RM et al. (2005) BMC Genomics "A gene expression fingerprint of C. elegans embryonic motor neurons."

(E). Posterior view of VNC showing F29G6.2::GFP expression in all VNC motor neurons. Asterisk marks gut autofluorescence (F). Scale bars are 10 microns in panel F and 20 microns in E.

Picture from Barkoulas M et al. (2016) PLoS Genet "Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene ...."

(B-E) smFISH using a lin-3 probe in C. elegans N2 (data from [2]) (B), C. briggsae AF16 (C), C. angaria RGD1 (D) and O. tipulae CEW1 (E). Red arrow marks the position of the anchor cell.

Picture from Senti G et al. (2008) Mol Biol Cell "Distinct isoforms of the RFX transcription factor DAF-19 regulate ciliogenesis ...."

An antibody specific for DAF-19A/B detects DAF-19 in all nonciliated neurons. (A) DAF-19 epitopes recognized by two different antibodies. Antibody AbDAF19N is specific for the long isoforms DAF-19A/B, whereas antibody AbDAF19C recognizes all three isoforms DAF-19A/B/C. (B-E) gfp reporter lines stained with antibodies against DAF-19. AbDAF19N detects DAF-19A/B only in nonciliated neurons (D and E) and not in ciliated sensory neurons (csn) (B and C). ceh-23::gfp marks ciliated sensory neurons, tph-1::gfp marks the serotonergic neuron ADF (ciliated), and nmr-1::gfp marks interneurons (nonciliated); see Supplemental Table 1 for details. (H) Schematic summary of all neurons investigated in the head region (blue, AbDAF19N; yellow, AbDAF19C; white, not determined neurons; and gray, no DAF-19 expression detected).

Picture from Fong Y et al. (2002) Science "Regulation of the different chromatin states of autosomes and X chromosomes in ...."

Figure 1. MES-4 in the germ line and in embryos and larvae. Samples were stained with either DAPI (4',6-diamidino-2-phenylindole) or antibodies to chromatin, and with anti-MES-4 (10). (A) Adult hermaphrodite gonad. (Upper panel) DAPI stain. (Lower panel) Anti-MES-4 stain. Asterisk marks the distal end, o marks oocytes. Bar, 50 um. (B) One-cell embryo in metaphase. (C) Six-cell, (D) ~80-cell, and (E) ~180-cell embryo. (F) L1 larva. Arrowhead points to the germline cells Z2 and Z3. Bar, 10 um (B) to (F).

Picture from Leacock SW et al. (2008) Genetics "MEG-1 and MEG-2 are embryo-specific P-granule components required for germline ...."

meg-1 is expressed in the proximal germline. In situ hybridization of meg-1 with antisense probe in wild-type gonad. No staining was observed with sense probe. Arrow marks onset of expression at mid-pachytene. Bar, 50 um.